Cre_lox位点特异性重组系统在高等真核生物中的研究进展_龙定沛.pdf

HEREDITAS (Beijing) 2012 2 , 34(2): 177―189 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2012.00177 Cre/lox 位点特异性重组系统在高等真核生物中的研究 进展 1 1,2 1 1,3 1 龙定沛 , 谭兵 , 赵爱春 , 许龙霞 , 向仲怀 1. , , 400716; 2. , , 430071; 3. , , 200031 来自于P1 噬菌体的Cre/lox 系统通过位点特异性重组可以迅速而有效地实现各种生理环境下的基因定点 插入、删除、替换和倒位等操作。Cre/lox 系统作为目前基因打靶技术的核心工具, 已被广泛应用于拟南芥、水 稻、小鼠、果蝇、斑马鱼等高等真核模式生物。文章较为全面地介绍了 Cre/lox 系统的基本概况及其在高等真 核生物中的应用, 讨论了 Cre/lox 系统在研究中存在的主要问题和今后的发展方向, 为利用该系统在不同高等 生物中进行基因操作提供有用的参考。 Cre/lox 重组系统; 基因操作; 位点特异性重组; 基因打靶 Progress in Cre/lox site-specific recombination system in higher eu- karyotes 1 1,2 1 1,3 1 LONG Ding-Pei , TAN Bing , ZHAO Ai-Chun , XU Long-Xia , XIANG Zhong-Huai 1. Institute of Sericulture and Systems Biology, Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China; 2. Molecular Virology and Viral Immunology Research Group, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; 3. Shanghai Institute of Biological Sciences, Institute of Biochemistry and Cell Biology, Shanghai 200031, China Abstract: Cre/lox system derived from P1 bacteriaphage can quickly and effectively achieve gene insertion, deletion, replacement, and inversion by means of site-specific recombination. As one of the most important tools for gene targeting at present, Cre/lox system has been widely used in Arabidopsis thaliana , Oryza sativa L., Mus musculus , Drosophila melanogaster, Danio