BiologEcoPlate_TM_实验信息提取方法改进_张燕燕_1.pdfVIP

微生物学通报 JUL 20, 2009, 36(7): 1083~1091 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 生物实验室 Biolog EcoPlateTM 实验信息提取方法改进 1,2 1 1* 张燕燕 曲来叶 陈利顶 (1. 100085) (2. 100049) 摘 要: Biolog EcoPlateTM 微平板是用于研究微生物群落水平多样性的一种主流研究方法。针对目 前国内研究者对Biolog 实验结果解读不充分和错误层出不穷的情况, 提出了一套改进实验结果信 息提取的方法。方法包含两个部分, 一是基于微生物生理学和生态学意义上的Biolog 碳源分类表, 是研究者进行解读的参考依据和索引; 二是结合实例, 阐释如何根据上述分类表、以环境富集原 理为指导, 利用排序的统计方法进行结果解译, 发掘出更多的信息。 关键词: Biolog, 群落水平多样性, 碳源, 分类, 解读 An Amendment on Information Extraction of Biolog EcoPlateTM ZHANG Yan-Yan1,2 QU Lai-Ye1 CHEN Li-Ding1* (1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China) (2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China) Abstract: Biolog EcoPlateTM is a mainstream approach of studying community-level physiological profiles of microbes. To correct misunderstanding and insufficiency of experimental result explanation, we an- nounced an amendment of information extraction of Biolog EcoPlateTM , which included two parts: (1) carbon substrates assortment sheets based on microbial physiological metabolism and ecological function, providing referrence and index for researchers; (2) illustration of how to use assorment sheet and ordination method to find out more information, guiding by environm