利用cDNA阵列初步发掘橡胶树抗寒相关基因.pdfVIP

热带作物学报 2016 ， 37(6): 1092-1097 Chinese Journal of Tropical Crops 利用 cDNA 阵列初步发掘橡胶树抗寒相关基因 程 汉，张燕燕，蔡海滨，安泽伟，胡彦师，黄华孙* 中国热带农业科学院橡胶研究所，海南倍州 571737 摘 要 通过橡胶树低温诱导全长 cDNA 文库，构建了 l 个含有 11708 个 Unigene 的 cDNA 阵列。使用这些 cDNA 阵列对橡胶树抗寒品种 93-114 和低温敏感种质材料热垦 501 进行基因表达谱研究。结果表明，在低温诱 导情况下，共有 84 个基因差异表达，其中 52 个上调表达， 32 个下调表达。在抗寒品种和低温敏感种质材料间 的差异基因有 21 个，其中在 93-114 中上洞的有 12 个，下调的有9 个。进一步对这些基因进行了功能注释。 关键词 橡胶树; cDNA 文库; cDNA 阵列;抗寒;差异基因 中图分类号 Q78; 5794.1 文献标识码 A A Preliminary Identitication of Cold Resistant Genes Using cDNA Arrays in Hevea brasiliensis CHENG Han , ZHANG Yanyan , CAI Haibin , AN Zewei , HU Yanshi , HUANG Huasun Rubber ReseOTch lnstitute, Chinese Academy 01 Trop町al Agricultural Scie时e， Donzho u, Hω， nan 571737, China Abstract The rubber tree cDNA arrays containing 11 708 unigenes were constructed using a cold inducing cDNA library. The aπays were used to study the gene expression in rubber tree cold resistant clone 93-114 and cold sensitive clone Reken 50 1. The results dernonstrated that 84 genes differentially expressed under cold treatrnent, in which 52 genes were up-regulated , while 32 down -re伊lated.ηle differentially expressing gene nurnber between 93-114 and Reken 501 was 21 , in which 12 genes were highly expressed and 9 lowly expressed in 93-114. 币lese genes were further functionally annotated.ηlÍs study provides ernpirical evidence for the developrnent of gene rnicroarray in rubber tree, and also contribute the data for the identification of cold resistant genes in Hevea br.由üiensis. Key words Hevea brasiliensis; cDNA library; cDNA aπay; Cold resistance; Differential gene doi 10.3969/j .issn.1α泊-2561.2016.06.∞7 转人单个抗寒基因往往达不到较好的抗寒效果。因 低温是影响植物分布、生长发育和产量的重要