抗生素筛选方法.pdfVIP

. 654 . 中国抗生素杂志2010年9月第35卷第9期 文章编号：1001-8689(2010)09-0654-05 抗生素筛选方法 1 2, * 1 马寅姣 宋沁馨 顾觉奋 (1 中国药科大学生命科学与技术学院，南京 210009 ； 2 中国药科大学药学院，南京 210009) 摘要：细菌耐药性发生率的升高，激励着人们去寻找更多的筛选抗生素的策略，进而发现了更多的抗生素作用机制。靶向 策略、高通量筛选等方法已经被用于检测有潜力的抗生素。细菌的DNA复制、细胞分裂和蛋白合成的中间步骤已经成为了筛选 的新靶点，双组分信号传递系统也成为了较为重要的药物新筛选靶点。微生物基因组学的发展，给新抗生素的发现带来了更大 的希望，使人们可以发现更多的新作用靶点。 关键词：抗生素；抗生素筛选；基因组；靶向抗生素筛选 中图分类号：R978.1 文献标识码：A Methods for the screening of antibiotics 1 2 1 Ma Yin-jiao , Song Qin-xin and Gu Jue-fen (1 School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009; 2 School of Pharmacy, China Pharmaceutical University, Nanjing 210009) Abstract The increasing incidence of bacterial drug-resistance has stimulated the development of strategies for screening new antibiotics methods and thus revealed many unexploited mechanisms about the action of antibiotic. Target-directed strategies, high-throughput screens and other methods were being used to detect potential antibacterial agents. Bacterial DNA replication, cell division and synthesis of protein were the targets of new screening methods; two-component signaling systems were also being targeted. The development of genomics has brought a prospect of fi nding new antibiotics and more and more new action targets will be discovered. Key words Antibiotic; Antibiotic screening; Genome; Target-directed antibiotic screening 目前，已经有许多结构各异、抗菌活性很好的 唑胺(linezolid) ；脂肽类的达托霉素(daptomycin)[1]]被 抗生素被