2009 蛋白质组学技术研究进展_刘军莲.pdfVIP

22 2 Vol. 22 No. 2 2009 4 Space edicine edicalEngineering Apr. 2009 蛋白质组学技术研究进展* 刘军莲,李勇枝, 高建义, 盖玉清, 王 静, 薛春美,辛冰牧 (, 100193) : 随着现代科学技术的飞速发展,现代生物学技术已经发展到后基因组时代, 包括人类在内的几十 生物体基因组测序已经完成,生命科学研究的重点从结构基因组研究转移到后基因组时代) ) ) 功能 基因组的研究,蛋白质组学研究是功能基因组一 个重要的组成部分因此, 与之相适应, 蛋白质组学研 究技术也是日新月异,层出不穷, 除了最经典的二维凝胶电泳技术, 多维液相色谱技术, 同位素标记亲和 标签技术相继发展起来,蛋白质芯片技术及噬菌体展示技术也得到了较为广泛的应用本文针对蛋白 质组学研究上述关键技术进行了综述 :蛋白质组学; 二维凝胶电泳; 多维液像色谱; 同位素标记亲和标签技术;蛋白质芯片;噬菌体展示 技术 : Q789 : A : 1002-0837(2009) 02-0151-06 The P rog ress of th e Proteom ic Technology. LI U Jun-lian, LI Yong-zh,i GAO Jian-y,i GA I Yu-qing, WANG J ing, XUE Chun-me,i X IN Bing-mu. S ace Medicine Medical Eng ineering, 2009, 22( 2): 151~ 156 Abs tract: W ith the ra di develo ment ofmodern scei nce and technology, the ost-genom ics eriod has comew ith the com lement of the sequence of body genome inc luding severa l tens of human genome, the em hasis of life science transfer from instruction genome to the ost-genomics er-i od, functiona lgenome. Proteom ics is the most mi ortant a rt of it. The technology of roteom ics is advanced day by day. Exce t the cal ssica l two dmi ens iona lgelelectro horesis, the technology of multi-dmi ensiona l liqudi chromatogra hy and the technology of isoto e coded-affinity tags have been successively develo ed, as well as rotein chi and hage dis lay have been a lei d extensively. This articel smi ly summarizes the key technologies of roteom ics. K ey w ord s: roteomics; two dmi ens iona lgelelectro horesis; mult-i dmi ensoi na l liquid chromatogra- hy; isoto e coded-affinity tags technology; rotein chi ; hage dis lay technology Address rep rin t requ ests to: LIU Jun-lai n. China Astronaut Resea rch and Training Cente,r Beijing 100193, China , , , ,