27 750-752 接738 食品安全与检测 3-10 高效液相色谱法测定大豆低聚糖中的棉籽糖和水苏糖.pdf

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.7 高效液相色谱法测定大豆低聚糖中的棉籽糖和水苏糖 王玉军，纪伟东，李永平，韩俊杰，叶红洋 (黑龙江粮食职业学院粮油工程系，黑龙江哈尔滨 150080) 摘要：建立了同时测定大豆低聚糖中棉籽糖和水苏糖含量的高效液相色谱分析方法（HPLC ）。采用示差折光检测器(RID) ，色谱柱为Agilent Zorbax carbohydrate柱(4.6 mm×150 mm, 5 μm)，流动相为乙腈-水(75:25, V:V) ，流速1.0 mL/min ，柱温30 ℃。实验结果表明：棉籽糖和水苏糖分别在1~10 mg/mL 、1~10 mg/mL 范围内线性良好，相关系数分别为0.9925 、0.9971；2种糖的相对标准偏差(RSD)分别为1.57%、1.81%(n=5)，平均回收率 分别为95.8%、96.0%。该分析方法简便，准确、可靠，适用于大豆低聚糖中棉籽糖和水苏糖含量的检测。 关键词：高效液相色谱；大豆低聚糖；棉籽糖；水苏糖 文章篇号：1673-9078(2010)7-750-752 Determination of Raffinose and Stachyose in Soybean Oligosaccharides by HPLC WANG Yu-jun, JI Wei-dong, LI Yong-ping, HAN Jun-jie, YE Hong-yang (HeiLongJiang Grain Vocational College, Harbin 150080, China) Abstract: A method was developed for the determination of raffinose and stachyose in soybean oligosaccharides by high performance liquid chromatography. The sample was separated on a Agilent Zorbax carbohydrate column (4.6 mm×150, 5 μm ) at 30 ℃ by RID and eluted with 75% acetonitrile aqueous solution at a rate of 1 mL/min. The results showed that the linear range for raffinose and stachyose, respectivelywere in the range of 1~10 mg /mL, 1~10 mg /mL with a correlation coefficient of 0.9925~0.9971.The relative standard deviations for raffinose and stachyose were 1.57%, 1.81% respectively (n=5) and the average recoveries were 95.8% and 96.0% respectively. Key words: HPLC; soybean oligosaccharides; raffinose; stachyose 750 大豆低聚糖(Soybean oligosaccharides, 1.1 仪器与试剂 SBOS)是大豆籽粒中可溶性寡糖的总称，主要 Agilent1100型高效液相色谱仪，配备四元 成分是水苏糖（stach