GeneticTechnology-ClarkCountySchoolDistrict基因技术-克拉克县学区.pptVIP

Genetic Technology 13.2 Recombinant DNA Genetic Engineering Selective breeding is one way to increase allele frequency Genetic engineering, or recombinant DNA is faster Genetic Engineering Genetic engineering = cutting DNA from one organism and inserting into another Recombinant DNA is made by connecting fragments from different sources Transgenic organisms contain foreign DNA Tobacco with firefly glow Genetic Engineering How is it done? Step 1: restriction enzymes cut DNA Each enzyme cuts a specific DNA segment Genetic Engineering How is it done? Step 1: restriction enzymes cut DNA Step 2: Vectors transfer DNA Vectors = virus, bacteria DNA or plasmid Vectors carry cut DNA segment into cell Genetic Engineering How is it done? Step 1: Restriction enzymes cut DNA Step 2: Vectors transfer DNA Step 3: Gene splicing Rejoining of DNA fragments DNA is copied when cell replicates Genetic Engineering Cloning Clones are genetically identical copies Dolly was the first mammal to be cloned from an adult cell, 1997 Cloning helps us understand how cells and genes work, may develop cures for diseases Applications of DNA technology Sequencing DNA – process to read nucleotide sequence Can help study of specific genes Applications of DNA technology Recombinant bacteria in industry Break down pollutants Recombinant DNA in medicine Create hormones, insulin Transgenic animals created to have human diseases for study