CAS平板覆盖法检测氢氧化细菌铁载体.PDFVIP

微生物学通报 Aug. 20, 2014, 41(8): 1692−1697 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.130462 生物实验室 CAS 平板覆盖法检测氢氧化细菌铁载体 1 1 2 1 1* 王卫星 周晓伦 李忠玲 王明鹏 王卫卫 (1. 710069) (2. 710600) 摘 要：【目的】用CAS 平板覆盖法检测氢氧化细菌铁载体，解决通用 CAS 琼脂平板法中十 六烷基三甲基溴化铵对真菌和某些细菌的生长抑制问题。【方法】将改良的CAS 检测培养基覆 盖在长满菌落的无铁培养基上，生长抑制问题因微生物未与十六烷基三甲基溴化铵直接接触而 解决。【结果】3 株氢氧化细菌 SDW-5、SDW-9 和AaP-13 均能产生单菌落，加入CAS 检测培 养基 1 h 后，菌落周围产生明显的铁载体晕圈。【结论】本方法成功解决了生长抑制问题，可以 作为检测微生物铁载体的通用方法。 关键词：覆盖，氢氧化细菌，铁载体 Detection of siderophore production from hydrogen-oxidizing bacteria with CAS overlay plate method WANG Wei-Xing1 ZHOU Xiao-Lun 1 LI Zhong-Ling2 WANG Ming-Peng 1 WANG Wei-Wei1* (1. The College of Life Sciences, Northwest University, Xi ’an, Shaanxi 710069, China) (2. Institute of Enzyme Engineering, Shannxi Academy of Sciences, Xi ’an, Shaanxi 710600, China) Abstract: [Objective] CAS (Chrome azurol S) overlay plate method was used for the detection of siderophore from hydrogen-oxidizing bacteria, to address the problem of growth inhibition for fungi and certain bacteria caused by hexadecyltrimethyl-ammonium bromide (HDTMA) in the popular CAS assay. [Methods] We just need to coverage the modified CAS medium on iron-free culture agar plates covered with colonies, then the problem of growth inhibition could be removed, due to the microorganisms tested with no direct contact with the hexadecyltrimethyl-amm