A Cyclin-Dependent Kinase that Promotes Cytokinesis through Modulating Phosphorylation of the Carboxy Terminal Domain of the RNA Pol II Rpb1p Sub-Unit 英文参考文献.docVIP

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A Cyclin-Dependent Kinase that Promotes Cytokinesis through Modulating Phosphorylation of the Carboxy Terminal Domain of the RNA Pol II Rpb1p Sub-Unit 英文参考文献.doc

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A Cyclin-Dependent Kinase that Promotes Cytokinesis through Modulating Phosphorylation of the Carboxy Terminal Domain of the RNA Pol II Rpb1p Sub-Unit 英文参考文献

ACyclin-DependentKinasethatPromotesCytokinesis throughModulatingPhosphorylationoftheCarboxy TerminalDomainoftheRNAPolIIRpb1pSub-Unit JimKaragiannis1¤*,MohanK.Balasubramanian1,2 1LaboratoryofCell Division, TemasekLifeSciencesLaboratory, Singapore, Singapore,2Department of BiologicalSciences,NationalUniversityof Singapore,Singapore,Singapore In Schizosaccharomyces pombe, the nuclear-localized kinase, Lsk1p, promotes cytokinesis by positively regulating the SeptationInitiationNetwork(SIN).Althoughamemberofthecyclin-dependentkinase(CDK)family,neitheracyclinpartner nor a physiological target has been identified. In this report we identify a cyclin, Lsc1p, that physically interacts and co- localizeswithLsk1p.Furthermore,lsk1D,lsc1D,aswellaskinase-deadlsk1-K306Rmutants,displayhighlysimilarcytokinesis defects. Lsk1p is related to CDKs that phosphorylate the carboxy-terminal domain (CTD) of the largest sub-unit of RNA polymeraseII(Rpb1p).Interestingly,wefindthatLsk1pandLsc1parerequiredforphosphorylationofSer-2residuesfoundin theheptadrepeatsoftheCTD.TodetermineifRpb1pcouldbeaphysiologicaltarget,wereplacedthenativerpb1genewith asyntheticgeneencodingaRpb1pproteininwhichSer-2wassubstitutedwiththenon-phosphorylatableamino-acidalanine inallheptads.Cellscarryingthisalleleweresimilartolsk1Dmutants:Theywereviable,displayedgeneticinteractionswiththe SIN, and were unable to complete cytokinesis upon perturbation of the cell division machinery. We conclude that Ser-2 phosphorylationoftheCTDheptadsplaysanovelphysiologicalroleintheregulationofcytokinesis. Citation: KaragiannisJ,BalasubramanianMK(2007)ACyclin-DependentKinasethatPromotesCytokinesisthroughModulatingPhosphorylationof theCarboxyTerminalDomainoftheRNAPolIIRpb1pSub-Unit.PLoSONE2(5):e433.doi:10.1371/journal.pone.0000433 INTRODUCTION phosphorylation status of the CTD influences initiation and elongationbythepolIIcomplex,aswellasthebindingofawide variety of accessory factors required for the proper splicing, capping,andpoly-a