A Molecular Insight into Algal-Oomycete Warfare cDNA Analysis of Ectocarpus siliculosus Infected with the Basal Oomycete Eurychasma dicksonii 英文参考文献.docVIP

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2017-05-11 发布于上海
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A Molecular Insight into Algal-Oomycete Warfare cDNA Analysis of Ectocarpus siliculosus Infected with the Basal Oomycete Eurychasma dicksonii 英文参考文献.doc

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A Molecular Insight into Algal-Oomycete Warfare cDNA Analysis of Ectocarpus siliculosus Infected with the Basal Oomycete Eurychasma dicksonii 英文参考文献

AMolecularInsightintoAlgal-OomyceteWarfare:cDNA AnalysisofEctocarpussiliculosusInfectedwiththeBasal OomyceteEurychasmadicksonii LauraGrenville-Briggs1.,ClaireM.M.Gachon2.,MartinaStrittmatter1,2,LievenSterck3,4,FrithjofC. Ku¨pper2,PietervanWest1* 1AberdeenOomyceteLaboratory,UniversityofAberdeen,Aberdeen,UnitedKingdom,2ScottishAssociationforMarineScience,ScottishMarineInstitute,Oban,Argyll, UnitedKingdom,3DepartmentofPlantSystemsBiology,FlandersInstituteforBiotechnology(VIB),Ghent,Belgium,4DepartmentofPlantBiotechnologyandGenetics, GhentUniversity,Ghent,Belgium Abstract Brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. Eurychasma dicksonii is anabundant,andprobably cosmopolitan,obligate biotrophic oomycete pathogenof marine brown algae. Oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic Stramenopiles, mostlyknownforcausingdevastatingagriculturalandaquaculturaldiseases.Whilstmolecularknowledgeisrestrictedto croppathogens,pathogenicoomycetesactuallyinfecthostsfrommosteukaryoticlineages.Molecularevidenceindicates that Eu. dicksonii belongs to the most early-branching oomycete clade known so far. Therefore Eu. dicksonii is of considerableinterestduetoitspresumedenvironmentalimpactandphylogeneticposition.Herewereportthefirstlarge scale functional molecular data acquired on the most basal oomycete to date. 9873 unigenes, totalling over 3.5Mb of sequencedata,wereproducedfromSanger-sequencedandpyrosequencedESTlibrariesofinfectedEctocarpussiliculosus. 6787unigenes(70%)wereofalgalorigin,and3086(30%)oomyceteorigin.57%ofEu.dicksoniisequenceshadnosimilarity topublishedsequencedata,indicatingthatthisdatasetislargelyunique.Wewereunabletopositivelyidentifysequences belongingtotheRXLRandCRNgroupsofoomyceteeffectorsidentifiedinhigheroomycetes,howeverweuncoveredother unique pathogenicity factors. These included putative algal cell wall degrading enzymes, cell surface proteins, and