A Novel Association between Two Trypanosome-Specific Factors and the Conserved L5-5S rRNA Complex 英文参考文献.docVIP

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A Novel Association between Two Trypanosome-Specific Factors and the Conserved L5-5S rRNA Complex 英文参考文献.doc

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A Novel Association between Two Trypanosome-Specific Factors and the Conserved L5-5S rRNA Complex 英文参考文献

ANovelAssociationbetweenTwoTrypanosome-Specific FactorsandtheConservedL5-5SrRNAComplex MartinCiganda,KimberlyProhaska,KristinaHellman,NoreenWilliams* DepartmentofMicrobiologyandImmunologyandWitebskyCenterforMicrobialPathogenesisandImmunology,UniversityatBuffalo,Buffalo,NewYork,UnitedStatesof America Abstract P34 and P37 are two previously identified RNA binding proteins in the flagellate protozoan Trypanosoma brucei . RNA interferencestudieshavedeterminedthattheproteinsareinvolvedinandessentialforribosomebiogenesis.Theproteins interact with the 5S rRNA with nearly identical binding characteristics. We have shown that this interaction is achieved mainlythroughtheLoopAregionoftheRNA,butP34andP37alsoprotecttheL5bindingsitelocatedonLoopC.Wenow provideevidencetoshowthatthesefactorsformanovelpre-ribosomalparticlethroughinteractionswithboth5SrRNA andtheL5ribosomalprotein.FurtherinsilicoandinvitroanalysisofT.bruceiL5indicatesaloweraffinityfor5SrRNAthan expected, based on other eukaryotic L5 proteins. We hypothesize that P34 and P37 complement L5 and bridge the interactionwith5SrRNA,stabilizingitandaidingintheearlystepsofribosomebiogenesis. Citation:CigandaM,ProhaskaK,HellmanK,WilliamsN(2012)ANovelAssociationbetweenTwoTrypanosome-SpecificFactorsandtheConservedL5-5SrRNA Complex.PLoSONE7(7):e41398.doi:10.1371/journal.pone.0041398 Editor:ZiyinLi,UniversityofTexas-HoustonMedicalSchool,UnitedStatesofAmerica ReceivedDecember19,2011;AcceptedJune25,2012;PublishedJuly31,2012 Copyright: ? 2012 Ciganda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbyNationalInstitutesofHealthGM092719.Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisionto publish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:nw1@ Intr