A Systematic Analysis of Cell Cycle Regulators in Yeast Reveals That Most Factors Act Independently of Cell Size to Control Initiation of Division 英文参考文献.docVIP

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A Systematic Analysis of Cell Cycle Regulators in Yeast Reveals That Most Factors Act Independently of Cell Size to Control Initiation of Division 英文参考文献.doc

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A Systematic Analysis of Cell Cycle Regulators in Yeast Reveals That Most Factors Act Independently of Cell Size to Control Initiation of Division 英文参考文献

ASystematicAnalysisofCellCycleRegulatorsinYeast RevealsThatMostFactorsActIndependentlyofCellSize toControlInitiationofDivision ScottA.Hoose1,JeremyA.Rawlings1,MichelleM.Kelly1,M.CamilleLeitch1,QotaibaO.Ababneh1, JuanP.Robles1,DavidTaylor1,EvelynM.Hoover1,BethelHailu1,KaylaA.McEnery1,S.Sabina Downing1,DeepikaKaushal1,YiChen1,AlexRife1,KirtanA.Brahmbhatt1,RogerSmithIII2 ,Michael Polymenis1* 1DepartmentofBiochemistryandBiophysics,TexasAMUniversity,CollegeStation,Texas,UnitedStatesofAmerica,2DepartmentofVeterinaryPathobiology,Texas AMUniversity,CollegeStation,Texas,UnitedStatesofAmerica Abstract Upstreameventsthattriggerinitiationofcelldivision,atapointcalledSTARTinyeast,determinetheoverallratesofcell proliferation.Theidentityandcompletesequenceofthoseeventsremainunknown.Previousstudiesreliedmainlyoncell sizechanges toidentify systematicallygenesrequiredforthetimelycompletionofSTART.Here,weevaluatedpanels of non-essentialsinglegenedeletionstrainsforalteredDNAcontentbyflowcytometry.Thisanalysisrevealedthatmostgene deletions that altered cell cycle progression did not change cell size. Our results highlight a strong requirement for ribosomalbiogenesisandproteinsynthesisforinitiationofcelldivision.Wealsoidentifiednumerousfactorsthathavenot been previously implicated in cell cycle control mechanisms. We found that CBS, which catalyzes the synthesis of cystathioninefromserineandhomocysteine,advancesSTARTintwoways:bypromotingcellgrowth,whichrequiresCBS’s catalyticactivity,andbyaseparatefunction,whichdoesnotrequireCBS’scatalyticactivity.CBSdefectscausediseasein humans,andinanimalsCBShasvital,non-catalytic,unknownroles.Hence,ourresultsmayberelevantforhumanbiology. Takentogether,thesefindingssignificantlyexpandtherangeoffactorsrequiredforthetimelyinitiationofcelldivision.The systematicidentificationofnon-essentialregulatorsofcelldivisionwedescribewillbeavaluableresourceforanalysisofcell cycleprogressioninyeastandotherorganisms. Citation:HooseSA,RawlingsJA,KellyMM,LeitchMC