A Riboswitch-Based Inducible Gene Expression System for Mycobacteria 英文参考文献.docVIP

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A Riboswitch-Based Inducible Gene Expression System for Mycobacteria 英文参考文献.doc

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A Riboswitch-Based Inducible Gene Expression System for Mycobacteria 英文参考文献

ARiboswitch-BasedInducibleGeneExpressionSystem forMycobacteria JessicaC.Seeliger1,ShanaTopp2,KimberlyM.Sogi2,MaryL.Previti1,JustinP.Gallivan5,CarolynR. Bertozzi2,3,4 * 1DepartmentofPharmacologicalSciences,StonyBrookUniversity,StonyBrook,NewYork,UnitedStatesofAmerica,2DepartmentsofChemistry,UniversityofCalifornia, Berkeley, California, United States of America, 3Molecular and Cell Biology, University of California, Berkeley, California, United States of America, 4Howard Hughes Medical Institute, University of California, Berkeley, California, United States of America, 5Department of Chemistry and the Center for Fundamental and Applied MolecularEvolution,EmoryUniversity,Atlanta,Georgia,UnitedStatesofAmerica Abstract Research on the human pathogen Mycobacterium tuberculosis (Mtb) would benefit from novel tools for regulated gene expression.Herewedescribethecharacterizationandapplicationofasyntheticriboswitch-basedsystem,whichcomprises a mycobacterial promoter for transcriptional control and a riboswitch for translational control. The system was used to induceandrepressheterologousproteinoverexpressionreversibly,tocreateaconditionalgeneknockdown,andtocontrol gene expression in a macrophage infection model. Unlike existing systems for controlling gene expression in Mtb , the riboswitchdoesnotrequiretheco-expressionofanyaccessoryproteins:alloftheregulatorymachineryisencodedbya short DNA segment directly upstream of the target gene. The inducible riboswitch platform has the potential to be a powerfulgeneralstrategyforcreatingcustomizedgeneregulationsystemsinMtb. Citation:SeeligerJC,ToppS,SogiKM,PrevitiML,GallivanJP,etal.(2012)ARiboswitch-BasedInducibleGeneExpressionSystemforMycobacteria.PLoSONE7(1): e29266.doi:10.1371/journal.pone.0029266 Editor:OlivierNeyrolles,InstitutdePharmacologieetdeBiologieStructurale,France ReceivedOctober19,2011;AcceptedNovember23,2011;PublishedJanuary18,2012 Copyright: ? 2012 Seeliger et al. This is an open-access article distributed under the