CharacterizationofProtein-Biomolecule-basedBiointerfaces.pptVIP

Characterization of Protein- Biomolecule-based Biointerfaces Prof. Prabhas Moghe November 13, 2006 125:583 Outline Methods for assessing concentration of biomolecules ELISA Radiolabeling Fluorometry Methods for assessing thickness, conformation, organization of biomolecules at interfaces Circular Dichroism FTIR Spectroscopy (Reviewed) Atomic force microscopy (Reviewed) Ellipsometry Total Internal Reflection Fluorescence (TIRF) (Reviewed) Methods for estimating protein or ligand affinity to interface Scatchard Analysis (Radiolabeling) Surface Plasmon Resonance (SPR) (Reviewed) Direct Determ