EnzymeKinetics.pptVIP

Table C.1-1. ____ Assay # Water (ml) Buffer Ethanol [S] NAD+ ADH V 1/V 1/[S] 1 0.000 0.700 2.100 0.100 0.100 2 0.600 0.700 1.500 0.100 0.100 3 1.100 0.700 1.000 0.100 0.100 4 1.600 0.700 0.500 0.100 0.100 5 1.900 0.700 0.200 0.100 0.100 6 2.000 0.700 0.100 0.100 0.100 7 2.050 0.700 0.050 0.100 0.100 8 2.080 0.700 0.020 0.100 0.100 9 2.090 0.700 0.010 0.100 0.100 10 2.095 0.700 0.005 0.100 0.100 Data table Page 87 Km Be careful 15 sec and 45 sec Read same and low = too little substrate Didn’t add enzyme Read same and high Reaction is over Contaminated one of your solutions with enzyme Did n