Proteinprecipitation.PDFVIP

Protein precipitation Acetone Precipitation Most universal Materials: • Sample of interest • Cold (-20°C) acetone, a volume four times that of the protein samples to be precipitated • Centrifuge tube • Centrifuge Method: 1. Cool the required volume of acetone to -20°C. 2. Add four times the sample volume of cold (-20°C) acetone to the tube. 3. Vortex tube and incubate for 60 minutes at -20°C. 4. Centrifuge 10 minutes at 13,000-15,000 x g. 5. Decant and properly dispose of the supernatant, being careful to not dislodge the pro