Activity-Dependent Changes in Cholinergic Innervation of the Mouse Olfactory Bulb 英文参考文献.docVIP
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Activity-Dependent Changes in Cholinergic Innervation of the Mouse Olfactory Bulb 英文参考文献
Activity-DependentChangesinCholinergicInnervation
oftheMouseOlfactoryBulb
ErnestoSalcedo1,TuanTran1,XuanLy1,RobertLopez1,CortneyBarbica1,DiegoRestrepo1,3 ,Sukumar
Vijayaraghavan2,3
*
1DepartmentofCellandDevelopmentalBiology,UniversityofColorado,SchoolofMedicine,Aurora,Colorado,UnitedStatesofAmerica,2DepartmentofPhysiologyand
Biophysics,UniversityofColorado,SchoolofMedicine,Aurora,Colorado,UnitedStatesofAmerica,3NeuroscienceProgram,UniversityofColorado,SchoolofMedicine,
Aurora,Colorado,UnitedStatesofAmerica
Abstract
Theinterplaybetweenolfactoryactivityandcholinergicmodulationremainstobefullyunderstood.Thisreportexamines
thepatternofcholinergicinnervationthroughoutthemurinemainolfactorybulbacross differentdevelopmentalstages
andinnaris-occludedanimals.Tovisualizethepatternofcholinergicinnervation,weusedatransgenicmousemodel,which
expressesafusionofthemicrotubule-associatedprotein,tau,withgreenfluorescenceprotein(GFP)underthecontrolof
the choline acetyltransferase (ChAT) promoter. This tau-GFP fusion product allows for a remarkably vivid and clear
visualizationofcholinergicinnervationinthemainolfactorybulb(MOB).Interestingly,wefindanunevendistributionof
GFP label in the adult glomerular layer (GL), where anterior, medial, and lateral glomerular regions of the bulb receive
relativelyheaviercholinergicinnervationthanotherregions.Incontrasttopreviousreports,wefindamarkedchangeinthe
patternofcholinergicinnervationtotheGLfollowingunilateralnaris occlusion betweentheipsilateralandcontralateral
bulbsinadultanimals.
Citation: Salcedo E,Tran T, Ly X,Lopez R, Barbica C, et al.(2011) Activity-Dependent Changes inCholinergic Innervation ofthe Mouse Olfactory Bulb. PLoS
ONE6(10):e25441.doi:10.1371/journal.pone.0025441
Editor:HiroakiMatsunami,DukeUniversity,UnitedStatesofAmerica
ReceivedApril15,2011;AcceptedSeptember5,2011;PublishedOctober28,2011
Copyright: ? 2011 Salcedo et al. This is an open-access article distributed under the terms of the Creative Commons Attributi
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