An Order of Magnitude Faster AIP1-Associated Actin Disruption than Nucleation by the Arp23 Complex in Lamellipodia 英文参考文献.docVIP

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An Order of Magnitude Faster AIP1-Associated Actin Disruption than Nucleation by the Arp23 Complex in Lamellipodia 英文参考文献.doc

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An Order of Magnitude Faster AIP1-Associated Actin Disruption than Nucleation by the Arp23 Complex in Lamellipodia 英文参考文献

AnOrderofMagnitudeFasterAIP1-AssociatedActin DisruptionthanNucleationbytheArp2/3Complexin Lamellipodia TakahiroTsuji,TakushiMiyoshi,ChiharuHigashida,ShuhNarumiya,NaokiWatanabe* DepartmentofPharmacology,KyotoUniversityFacultyofMedicine,Kyoto,Japan Abstract The mechanism of lamellipod actin turnover is still under debate. To clarify the intracellular behavior of the recently- identifiedactindisruptionmechanism,weexaminedkineticsofAIP1usingfluorescentsingle-moleculespecklemicroscopy. AIP1isthoughttocapcofilin-generatedactinbarbedends.Herewedemonstrateareductioninactin-associatedAIP1in lamellipodia of cells overexpressing LIM-kinase. Moreover, actin-associated AIP1 was rapidly abolished by jasplakinolide, which concurrently blocked the F-actin-cofilin interaction. Jasplakinolide also slowed dissociation of AIP1, which is analogoustotheeffectofthisdrugoncappingprotein.ThesefindingsprovideinvivoevidenceoftheassociationofAIP1 withbarbedendsgeneratedbycofilin-catalyzedfilamentdisruption.Single-moleculeobservationfounddistributionofF- actin-associated AIP1 throughout lamellipodia, and revealed even faster dissociation of AIP1 than capping protein. The estimatedoverallAIP1-associatedactindisruptionrate,1.8mM/s,wasoneorderofmagnitudefasterthanArp2/3complex- catalyzedactinnucleationinlamellipodia.Thisratedoesnotsufficethefilamentseveringratepredictedinourprevious highfrequencyfilamentsevering-annealinghypothesis.Ourdatatogetherwithrecentbiochemicalstudiesimplybarbed end-preferredfrequentfilamentdisruption.FrequentgenerationofAIP1-associatedbarbedendsandsubsequentreleaseof AIP1maybethemechanismthatfacilitatespreviouslyobservedubiquitousactinpolymerizationthroughoutlamellipodia. Citation:TsujiT,MiyoshiT,HigashidaC,NarumiyaS,WatanabeN(2009)AnOrderofMagnitudeFasterAIP1-AssociatedActinDisruptionthanNucleationbythe Arp2/3ComplexinLamellipodia.PLoSONE4(3):e4921.doi:10.1371/journal.pone.0004921 Editor:NeilHotchin,UniversityofBirmingham,UnitedKingdom ReceivedNovember21,2008;AcceptedFe