An In Vivo Transfection Approach Elucidates a Role for Aedes aegypti Thioester-Containing Proteins in Flaviviral Infection 英文参考文献.docVIP

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An In Vivo Transfection Approach Elucidates a Role for Aedes aegypti Thioester-Containing Proteins in Flaviviral Infection 英文参考文献.doc

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An In Vivo Transfection Approach Elucidates a Role for Aedes aegypti Thioester-Containing Proteins in Flaviviral Infection 英文参考文献

AnInVivoTransfectionApproachElucidatesaRolefor AedesaegyptiThioester-ContainingProteinsinFlaviviral Infection GongCheng1,LeiLiu1,PenghuaWang1,YueZhang1,YangO.Zhao1,TonyaM.Colpitts1 ,Fabiana Feitosa1,JohnF.Anderson2,ErolFikrig1,3* 1SectionofInfectiousDiseases,DepartmentofInternalMedicine,YaleUniversitySchoolofMedicine,NewHaven,Connecticut,UnitedStatesofAmerica,2Departmentof Entomology,ConnecticutAgriculturalExperimentStation,NewHaven,Connecticut,UnitedStatesofAmerica,3HowardHughesMedicalInstitute,ChevyChase,Maryland, UnitedStatesofAmerica Abstract Mosquitoestransmitpathogensthatcauseinfectiousdiseasesofglobalimportance.Techniquestoeasilyintroducegenes intomosquitoes,however,limitinvestigationsoftheinteractionbetweenmicrobesandtheirarthropodvectors.Wenow show that a cationic liposome significantly enhances delivery and expression of plasmid DNA in Aedes aegypti and Anophelesgambiaemosquitoes.WethenintroducedthegenesforAe.aegyptithioester-containingproteins(AeTEPs),which areinvolvedinthecontrolofflaviviralinfection,intomosquitoesusingthistechnique.InvivotransfectionofAeTEP-1into Ae. aegypti significantly reduced dengue virus infection, suggesting that the approach can further our understanding of pathogen-mosquitointeractions. Citation:ChengG,LiuL,WangP,ZhangY,ZhaoYO,etal.(2011)AnInVivoTransfectionApproachElucidatesaRoleforAedesaegyptiThioester-Containing ProteinsinFlaviviralInfection.PLoSONE6(7):e22786.doi:10.1371/journal.pone.0022786 Editor:AliAmara,InstitutUniversitaired’He′matologie,France ReceivedFebruary10,2011;AcceptedJuly4,2011;PublishedJuly27,2011 Copyright: ? 2011 Cheng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:E.F.isaninvestigatorwiththeHowardHughesMedicalInstitute.G.C.isaJamesHudsonBrown-AlexanderBrownCoxePostdoctoralFellowinYale SchoolofMedicine.P.W.issupportedby