Analysis of Differential miRNA Expression in the Duodenum of Escherichia coli F18-Sensitive and -Resistant Weaned Piglets 英文参考文献.docVIP

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Analysis of Differential miRNA Expression in the Duodenum of Escherichia coli F18-Sensitive and -Resistant Weaned Piglets 英文参考文献.doc

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Analysis of Differential miRNA Expression in the Duodenum of Escherichia coli F18-Sensitive and -Resistant Weaned Piglets 英文参考文献

AnalysisofDifferentialmiRNAExpressioninthe DuodenumofEscherichiacoliF18-Sensitive and-ResistantWeanedPiglets LanYe1,XianminSu1,ZhengchangWu1,XianruiZheng1,JinWang1,ChenZi1,GuoqiangZhu2, ShenglongWu1*,WenbinBao1* 1KeyLaboratoryforAnimalGenetics,Breeding,ReproductionandMolecularDesignofJiangsuProvince,CollegeofAnimalScienceandTechnology,YangzhouUniversity, Yangzhou,JiangsuProvince,China,2CollegeofVeterinaryMedicine,YangzhouUniversity,Yangzhou,JiangsuProvince,China Abstract SmallRNAduodenallibrarieswereconstructedforEscherichiacoliF18-sensitiveand-resistantweanedpigletsinfull-sibpair groups andsequencedusing IlluminaSolexa high-throughput sequencing technology. Theidentification of differentially expressedmiRNAsprovidesthebasisforimproveddatabaseinformationonpigmiRNAs,understandingthegeneticbasics ofdifferencesinresistancetoE.coliF18betweenlocalChineseandexoticpigbreeds,andfindingnewresistancemarkers forE.coliF18infection.Theduodenumofallindividualscontainedmorethan90%ofknownswinemiRNAs.Atotalof58 differentiallyexpressingmiRNAswereidentified,ofwhich46wereincreasedand12weredecreasedinE.coliF18-sensitive pigs.OfmiRNAswithincreasedexpression,ssc-miR-143wasmosthighlyexpressed,followedbyssc-let-7f,ssc-miR-192,and ssc-miR-21. We identified a total of 2036 intersection target genes by comparing TargetScan data and previous gene expressionprofileresults.Geneontologyandpathwayanalysisofintersectiongenesshowedthatdifferentiallyexpressed miRNAsweremainlyinvolvedintheimmuneresponseandtranscriptionalregulation.Combininginformationondifferential miRNA expression and their regulatory relationships with transcription factors, identified 12 candidate miRNA disease markers,including11miRNAswithincreasedexpression,ssc-miR-143,ssc-let-7f,ssc-miR-30e,ssc-miR-148a,ssc-miR-148b, ssc-miR-181a,ssc-miR-192,ssc-miR-27b,ssc-miR-15b,ssc-miR-21,andssc-miR-215,andonewithdecreasedexpression,ssc- miR-152.Quantitativereal-timePCRanalysisofcandidatemiRNAexpressioninalargercohortofEcoliF18-sensitivean