Antibiotic Susceptibility Testing of Grown Blood Cultures by Combining Culture and Real-Time Polymerase Chain Reaction Is Rapid and Effective 英文参考文献.docVIP
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Antibiotic Susceptibility Testing of Grown Blood Cultures by Combining Culture and Real-Time Polymerase Chain Reaction Is Rapid and Effective 英文参考文献
AntibioticSusceptibilityTestingofGrownBloodCultures
byCombiningCultureandReal-TimePolymeraseChain
ReactionIsRapidandEffective
JudithBeuving,AnneliesVerbon,FirzaA.Gronthoud,EllenE.Stobberingh,PetraF.G.Wolffs*
DepartmentofMedicalMicrobiology,CareAndPublicHealthResearchInstitute(CAPHRI),MaastrichtUniversityMedicalCenter,Maastricht,TheNetherlands
Abstract
Background:Earlyadministrationofappropriateantibiotictherapyinbacteraemiapatientsdramaticallyreducesmortality.
AnewmethodforRApidMolecularAntibioticSusceptibilityTesting(RAMAST)thatcanbeapplieddirectlytopositiveblood
cultureswasdevelopedandevaluated.
Methodology/Principal Findings: Growth curves and antibiotic susceptibility of blood culture isolates (Staphylococcus
aureus, enterococci and (facultative) aerobic Gram-negative rods) were determined by incubating diluted blood cultures
withandwithoutantibiotics,followedbyaquantitativeuniversal16SPCRtodetectthepresenceorabsenceofgrowth.
Testing114positivebloodcultures,RAMASTshowedanagreementwithmicrobrothdilutionof96.7%forGram-negative
rods,withaminorerror(false-susceptibilitywithaintermediateresistantstrain)rateof1.9%,amajorerror(falseresistance)
rateof0.8%andaverymajorerror(falsesusceptibility)rateof0.6%.AgreementforS.aureuswas97.9%,withaverymajor
errorrateof2.1%.Enterococcusspeciesshowed95.0%agreement,withamajorerrorrateof5.0%.Theseagreementsare
comparable with those of the Phoenix system. Starting from a positive blood culture, the test was completed within
9hours.
Conclusions/Significance: This new rapid method for antibiotic susceptibility testing can potentially provide accurate
resultsformostrelevantbacteriacommonlyisolatedfrompositivebloodculturesinlesstimethanroutinemethods.
Citation:BeuvingJ,VerbonA,GronthoudFA,StobberinghEE,WolffsPFG(2011)AntibioticSusceptibilityTestingofGrownBloodCulturesbyCombiningCulture
andReal-TimePolymeraseChainReactionIsRapidandEffective.PLoSONE6(12):e27689.doi:10.1371/journal.pone.0027689
Editor:GunnarF.Kaufmann,TheScrippsResearchInst
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