Antigenic Characterization of Recombinant Hemagglutinin Proteins Derived from Different Avian Influenza Virus Subtypes 英文参考文献.docVIP
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Antigenic Characterization of Recombinant Hemagglutinin Proteins Derived from Different Avian Influenza Virus Subtypes 英文参考文献
AntigenicCharacterizationofRecombinant
HemagglutininProteinsDerivedfromDifferentAvian
InfluenzaVirusSubtypes
MatthiasMueller*,SandraRenzullo,RoxannBrooks,NicolasRuggli,MartinA.Hofmann
InstituteofVirologyandImmunoprophylaxis(IVI),Mittelhaeusern,Switzerland
Abstract
Sincetheadventofhighlypathogenicvariantsofavianinfluenzavirus(HPAIV),themainfocusofavianinfluenzaresearch
hasbeenthecharacterizationanddetectionofHPAIVhemagglutinin(HA)fromH5andH7subtypes.However,duetothe
highmutationandreassortationrateofinfluenzaviruses,intheoryanyinfluenzastrainmayacquireincreasedpathogenicity
irrespectiveofitssubtype.Acomprehensiveantigeniccharacterizationofinfluenzavirusesencompassingall16HAand9
neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly,
vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus
system.Theseproteinswereusedtogeneratepolyclonalrabbitantisera,whichweresubsequentlyemployedinepitope
scanninganalysisusingpeptidelibrariesspanningtheentireHA.Here,wereporttheidentificationandcharacterizationof
linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HAproteins. Selected subtype-specific
epitopeswereshowntobesuitableforthedifferentiationofanti-HAantibodiesinanELISA.
Citation: Mueller M, Renzullo S, Brooks R, Ruggli N, Hofmann MA (2010) Antigenic Characterization of Recombinant Hemagglutinin Proteins Derived from
DifferentAvianInfluenzaVirusSubtypes.PLoSONE5(2):e9097.doi:10.1371/journal.pone.0009097
Editor:YuWang,HongKongUniversity,HongKong
ReceivedOctober19,2009;AcceptedJanuary19,2010;PublishedFebruary5,2010
Copyright: ? 2010 Mueller et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited.
Funding: The study was funded by the Swiss Federal Veterinary Of
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