Antimetastatic Effects of Phyllanthus on Human Lung (A549) and Breast (MCF-7) Cancer Cell Lines 英文参考文献.docVIP

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Antimetastatic Effects of Phyllanthus on Human Lung (A549) and Breast (MCF-7) Cancer Cell Lines 英文参考文献.doc

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Antimetastatic Effects of Phyllanthus on Human Lung (A549) and Breast (MCF-7) Cancer Cell Lines 英文参考文献

AntimetastaticEffectsofPhyllanthusonHumanLung (A549)andBreast(MCF-7)CancerCellLines SauHarLee1,InduBalaJaganath2,SeokMuiWang1,ShamalaDeviSekaran1* 1Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia, 2Biotechnology Centre, Malaysia Agricultural Research and DevelopmentInstitute(MARDI),Serdang,Malaysia Abstract Background: Current chemotherapeutic drugs kill cancer cells mainly by inducing apoptosis. However, they become ineffectiveoncecancercellhastheabilitytometastasize,hencethepoorprognosisandhighmortalityrate.Therefore,the purposeofthisstudywastoevaluatetheantimetastaticpotential of Phyllanthus(P. niruri,P.urinaria,P.watsonii,andP. amarus)onlungandbreastcarcinomacells. Methodology/Principal Findings: Cytotoxicity of Phyllanthus plant extracts were first screened using the MTS reduction assay. They were shown to inhibit MCF-7 (breast carcinoma) and A549 (lung carcinoma) cells growth with IC values 50 rangingfrom50–180mg/mland65–470mg/mlformethanolicandaqueousextractsrespectively.Incomparison,theyhave lowertoxicityonnormalcellswiththecellviabilitypercentageremainingabove50%whentreatedupto1000 mg/mlfor bothextracts.Afterdeterminingthenon-toxiceffectivedose,severalantimetastasisassayswerecarriedoutandPhyllanthus extracts were shown to effectively reduce invasion, migration, and adhesion of both MCF-7 and A549 cells in a dose- dependentmanner,atconcentrationsrangingfrom20–200mg/mlformethanolicextractsand50–500 mg/mlforaqueous extracts.Thiswasfollowedbyanevaluationofthepossiblemodesofcelldeaththatoccurredalongwiththeantimetastatic activity. Phyllanthus was shown to be capable of inducing apoptosis in conjunction with its antimetastastic action, with morethanthreefoldincreaseofcaspases-3and-7,thepresenceofDNA-fragmentationandTUNEL-positivecells.Theability of Phyllanthus to exert antimetastatic activities is mostly associated to the presence of polyphenol compounds in its extracts. Conclusions/Signific