Assembly of Microtubules and Actomyosin Rings in the Absence of Nuclei and Spindle Pole Bodies Revealed by a Novel Genetic Method 英文参考文献.docVIP
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Assembly of Microtubules and Actomyosin Rings in the Absence of Nuclei and Spindle Pole Bodies Revealed by a Novel Genetic Method 英文参考文献
AssemblyofMicrotubulesandActomyosinRingsinthe
AbsenceofNucleiandSpindlePoleBodiesRevealedby
aNovelGeneticMethod
YinyiHuang1,2,P.T.Tran3,SnezhanaOliferenko1,MohanK.Balasubramanian1,2*
1Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 2Department of Biological Sciences, National University of
Singapore, Singapore, 3Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of
America
Background. The nucleus and the centrosomes (spindle pole bodies; SPBs in yeast) are believed to play key roles in the
organizationofvariouscellularstructures,suchastheactomyosinringandmicrotubules.Theabilitytogeneratecellslacking
nuclei and centrosomes (SPBs) is key to the elucidation of the role of these structures in various cellular processes.
Methodology/Principal Findings. Here we describe a genetic method, using the Schizosaccharomyces pombe cdc16-116
mutant,toreliablyandefficientlygeneratefissionyeastcellslackingnucleiandSPBs.Weusethisapproachtoshowthatthe
assemblyofmicrotubules doesnotrequirenuclearassociatedmicrotubuleorganizing centersandSPBs.Wealsoshowthat
actomyosin rings can assemble albeit inefficiently in the absence of nuclei and SPBs. Conclusion. We conclude that key
cytoskeletal elements can be assembled in the absence of nuclei and SPBs. In addition, the approach we describe, taken
together with physical approaches such as centrifugation, should facilitate the investigation ofthe role ofthe nucleus and
SPBsintheassemblyandinheritanceofvariouscellularstructuresandorganelles.
Citation: HuangY,TranPT,OliferenkoS,BalasubramanianMK(2007)AssemblyofMicrotubulesandActomyosinRingsintheAbsenceofNucleiand
SpindlePoleBodiesRevealedbyaNovelGeneticMethod.PLoSONE2(7):e618.doi:10.1371/journal.pone.0000618
INTRODUCTION
complex [13]. Loss of function of the cdc16 gene leads to
constitutiveSINsignalingandmultiplewavesofactomyosinring
assemblyandseptation[14]evenintheabsenceofmitosis[15].As
aresu
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