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Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development 英文参考文献
Aureliaaurita(Cnidaria)Oocytes’ContactPlateStructure
andDevelopment
LeonidS.Adonin1,2,TatyanaG.Shaposhnikova2,OlgaPodgornaya1,2*
1Institute of Cytology RAS, St. Petersburg, Russia, 2Department of Cytology and Histology, Faculty of Biology and Soil Sciences, St-Petersburg State University, St.
Petersburg,Russia
Abstract
OneoftheA.auritamedusamainmesogleapolypeptides,mesoglein,hasbeendescribedpreviously.Mesogleinbelongsto
ZP-domainproteinfamilyandthereforewefocusedonA.auritaoogenesis.Antibodiesagainstmesoglein(ABRA47)stain
theplateintheplacewheregerminalepitheliumcontactsoocyteontheparaffinsections.Accordingtoitsposition,we
namedthestructurefoundthe‘‘contactplate’’.OurmaininstrumentwasABagainstmesoglein.ZP-domainoccupiesabout
halfofthewholeaminoacidsequenceofthemesoglein.ImmunoblotafterSDSandAUrevealstwocharged
and high Mr bands among the female gonad germinal epithelium polypeptides. One of the gonads’ polypeptides Mr
corresponds to that of mesogleal cells, the other ones’ Mr is higher. The morphological description of contact plate
formationisthesubjectofthecurrentwork.Twotypes ofABRA47positivegranuleswereobservedduringprogressive
oogenesisstages.Granulesformthecontactplateinmatureoocyte.ContactplateofA.auritaoocytemarksitsanimalpole
andresemblesZonaPellucidabythefollowingfeatures:(1)itattractsspermatozoids;(2)thematerialofthecontactplateis
synthesizedbyoocyteandstoredingranules;(3)thesegranulesandthecontactplateitselfcontainZPdomainprotein(s);
(4)contactplateisanextracellularstructuremadeupoffiberbundlessimilartothoseofconventionalZonaPellucida.
Citation: Adonin LS, Shaposhnikova TG, Podgornaya O (2012) Aurelia aurita (Cnidaria) Oocytes’ Contact Plate Structure and Development. PLoS ONE 7(11):
e46542.doi:10.1371/journal.pone.0046542
Editor:ChristianBo¨kel,TechnischeUniversita¨t Dresden,Germany
ReceivedMay23,2012;AcceptedAugust31,2012;PublishedNovember21,2012
Copyright: ? 2012 Adonin et al. This is an open-access article distributed under the terms of the C
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