Binding Leverage as a Molecular Basis for Allosteric Regulation 英文参考文献.docVIP

下载本文档

3
0
约6.32万字
约 13页
2017-05-11 发布于上海
举报
版权申诉

Binding Leverage as a Molecular Basis for Allosteric Regulation 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Binding Leverage as a Molecular Basis for Allosteric Regulation 英文参考文献

BindingLeverageasaMolecularBasisforAllosteric Regulation SimonMitternacht1,2,IgorN.Berezovsky1* 1ComputationalBiologyUnit/UNIResearch,UniversityofBergen,Bergen,Norway,2DepartmentofInformatics,UniversityofBergen,Bergen,Norway Abstract Allostericregulationinvolvesconformationaltransitionsorfluctuationsbetweenafewcloselyrelatedstates,causedbythe bindingofeffectormolecules.Weintroduceaquantitycalledbindingleveragethatmeasurestheabilityofabindingsiteto coupletotheintrinsicmotionsofaprotein.WeuseMonteCarlosimulationstogeneratepotentialbindingsitesandeither normal modes or pairs of crystal structures to describe relevant motions. We analyze single catalytic domains and multimericallostericenzymeswithcomplexregulation.Forthemajorityoftheanalyzedproteins,wefindthatbothcatalytic and allosteric sites have high binding leverage. Furthermore, our analysis of the catabolite activator protein, which is allostericwithoutconformationalchange,showsthatitsregulationinvolvesothertypesofmotionthanthosemodulatedat siteswithhighbindingleverage.Ourresultspointtotheimportanceofincorporatingdynamicinformationwhenpredicting functional sites. Because it is possible to calculate binding leverage from a single crystal structure it can be used for characterizingproteinsofunknownfunctionandpredictinglatentallostericsitesinanyprotein,withimplicationsfordrug design. Citation: Mitternacht S,BerezovskyIN (2011) Binding Leverage asa Molecular Basis for Allosteric Regulation. PLoS Comput Biol 7(9): e1002148. doi:10.1371/ journal.pcbi.1002148 Editor:ThomasLengauer,Max-Planck-Institutfu¨rInformatik,Germany ReceivedApril5,2011;AcceptedJune21,2011;PublishedSeptember15,2011 Copyright: ? 2011 Mitternacht, Berezovsky. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisstudywasfundedbytheNorwegianResearchCouncil.Thefundershadnorole