BRITER A BMP Responsive Osteoblast Reporter Cell Line 英文参考文献.docVIP

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BRITER A BMP Responsive Osteoblast Reporter Cell Line 英文参考文献.doc

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BRITER A BMP Responsive Osteoblast Reporter Cell Line 英文参考文献

BRITER:ABMPResponsiveOsteoblastReporterCellLine PremSwaroopYadav.,ParitoshPrashar.,AmitabhaBandyopadhyay* DepartmentofBiologicalSciencesandBioengineering,IndianInstituteofTechnology,Kanpur,India Abstract Background: BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput moleculargeneticscreeningmayrevealnovelplayersregulatingBMPsignalingresponsewhilechemicalgeneticscreening ofBMPsignalingmodifiersmayhaveclinicalsignificance.Itisthereforeimportanttogenerateacell-basedtooltoexecute suchscreens. Methodology/Principal Findings: We have established a BMP responsive reporter cell line by stably integrating a BMP responsivedualluciferasereporterconstructintheimmortalizedcalvarialosteoblastcellsisolatedfromtamoxifeninducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to addedBMPmaybefurtherincreasedbydepletingtheendogenousBMP2andBMP4proteins. Conclusion: As the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specificallyandpromptlytoexogenouslyaddedBMP2protein,BRITERmaybeusedeffectivelyforchemicalormolecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signalingpathwaymayhaveclinicalsignificance. Citation: Yadav PS, Prashar P, Bandyopadhyay A (2012) BRITER: A BMP Responsive Osteoblast Reporter Cell Line. PLoS ONE 7(5): e37134. doi:10.1371/ journal.pone.0037134 Editor:AustinJohnCooney,BaylorCollegeofMedicine,UnitedStatesofAmerica ReceivedDecember10,2011;AcceptedApril13,2012;PublishedMay14,2012 Copyright: ? 2012 Yadav et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcear