Cajaninstilbene Acid Relaxes Rat Renal Arteries Roles of Ca2+ Antagonism and Protein Kinase C-Dependent Mechanism 英文参考文献.docVIP

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Cajaninstilbene Acid Relaxes Rat Renal Arteries Roles of Ca2+ Antagonism and Protein Kinase C-Dependent Mechanism 英文参考文献.doc

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CajaninstilbeneAcidRelaxesRatRenalArteriesRolesofCa2AntagonismandProteinKinaseC-DependentMechanism英文参考文献

CajaninstilbeneAcidRelaxesRatRenalArteries:Rolesof Ca AntagonismandProteinKinaseC-Dependent Mechanism 2+ Dong-MeiZhang1,2.,YongLi1,2.,WaiSanCheang3.,ChiWaiLau3,Shun-MingLin1,2,Qian-LanZhang1,2 , NanYao1,2,YingWang1,2,XinWu1,2,YuHuang3*,Wen-CaiYe1,2* 1Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, China, 2Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou, China, 3Institute of Vascular Medicine, Li Ka ShingInstituteofHealthSciencesandSchoolofBiomedicalSciences,ChineseUniversityofHongKong,HongKong,China Abstract Cajaninstilbeneacid(CSA)isamajoractivecomponentpresentintheleavesofCajanuscajan(L.)Millsp.Thepresentstudy explores the underlying cellular mechanisms for CSA-induced relaxation in rat renal arteries. Vascular reactivity was examinedinarterialringsthatweresuspendedinaMultiMyographSystemandtheexpressionofsignalingproteinswas assessed by Western blotting method. CSA (0.1–10mM) produced relaxations in rings pre-contracted by phenylephrine, serotonin, 9,11-dideoxy-9a,11a-epoxymethanoprostaglandin F2a (U46619),and60mM KCl.CSA-inducedrelaxationsdid notshowdifferencebetweengendersandwereunaffectedbyendotheliumdenudation,norbytreatmentwithNG-nitro-L- arginine methyl ester, indomethacin, ICI-182780, tetraethylammonium ion, BaCl2, glibenclamide, 4-aminopyridine or 2+ propranolol.CSAreducedcontractioninducedbyCaCl2(0.01–5mM)inCa -free60mMKClsolutionandby30nM(2)-Bay K8644in15mMKClsolution.CSAinhibited60mMKCl-inducedCa influxinsmoothmuscleofrenalarteries.Inaddition, CSA inhibited contraction evoked by phorbol 12-myristate 13-acetate (PMA, protein kinase C agonist) in Ca -free Krebs 2+ 2+ solution.Moreover,CSAreducedtheU46619-andPMA-inducedphosphorylationofmyosinlightchain(MLC)atSer19and myosinphosphatasetargetsubunit1(MYPT1)atThr853whichwasassociatedwithvasoconstriction.CSAalsoloweredthe phosphory