CDK11p58 Is Required for Centriole Duplication and Plk4 Recruitment to Mitotic Centrosomes 英文参考文献.docVIP

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CDK11p58 Is Required for Centriole Duplication and Plk4 Recruitment to Mitotic Centrosomes 英文参考文献.doc

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CDK11p58 Is Required for Centriole Duplication and Plk4 Recruitment to Mitotic Centrosomes 英文参考文献

CDK11p58 IsRequiredforCentrioleDuplicationandPlk4 RecruitmenttoMitoticCentrosomes NathalieFranck.,EmilieMontembault.¤,PierreRome′,AudePascal,Jean-YvesCremet,Re′gisGiet* UMR6061-CNRS,InstitutdeGe′ne′tiqueetDe′veloppementdeRennes,Universite′ deRennesI,Rennes,France Abstract Background:CDK11p58isamitoticproteinkinase,whichhasbeenshowntoberequiredfordifferentmitoticeventssuchas centrosomematuration,chromatidcohesionandcytokinesis. Methodology/PrincipalFindings:Inadditiontothesepreviouslydescribedroles,ourstudyshowsthatCDK11p58 inhibition inducesafailureinthecentrioleduplicationprocessindifferenthumancelllines.Weproposethatthiseffectismediatedby thedefectivecentrosomalrecruitmentofproteinsattheonsetofmitosis.Indeed,Plk4proteinkinaseandthecentrosomal proteinCep192,whicharekeycomponentsofthecentrioleduplicationmachinery,showedreducedlevelsatcentrosomes ofmitoticCDK11-depletedcells.CDK11p58,whichaccumulatesonlyinthevicinityofmitoticcentrosomes,directlyinteracts withthecentriole-associatedproteinkinasePlk4thatregulatescentriolenumberincells.Inaddition,weshowthatcentriole fromCDK11defectivecellsarenotabletobeoverduplicatedfollowingPlk4overexpression. Conclusion/Significance:WethusproposethatCDK11isrequiredforcentrioleduplicationbytwonon-mutually-exclusive mechanisms.Ononehand,theobservedduplicationdefectcouldbecausedindirectlybyafailureofthecentrosometo fullymaturateduringmitosis.Ontheotherhand,CDK11p58couldalsodirectlyregulatekeycentriolecomponentssuchas Plk4duringmitosistotriggeressentialmitoticcentriolemodifications,requiredforcentrioleduplicationduringsubsequent interphase. Citation:FranckN,MontembaultE,Rome′ P,PascalA,CremetJ-Y,etal.(2011)CDK11p58 IsRequiredforCentrioleDuplicationandPlk4RecruitmenttoMitotic Centrosomes.PLoSONE6(1):e14600.doi:10.1371/journal.pone.0014600 Editor:NeilA.Hotchin,UniversityofBirmingham,UnitedKingdom ReceivedJuly16,2010;AcceptedDecember20,2010;PublishedJanuary31,2011 Copyright: ? 2011 Franck et al. This is an open-access article dis