填充床反应器中酶法合成生物蜡酯.pdfVIP

学兔兔 化 丁 进 展 ·864· CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2009年第28卷第5期 填充床反应器中酶法合成生物蜡酯 刘婷婷，邓 利，鲁吉珂，谭天伟，王 芳 (北京化上大学生命科学与技术学院，北京市生物加工过程重点实验室生物炼制教育部工程 研究中心，北京 100029) 摘 要：研究了填充床式反应器酶法合成生物蜡酯的生产_Y-艺。以大豆油、十六醇为原料，在石油醚体系中，使 用实验室自制的固定化Candida sp．99—125为催化剂。对操作参数，如填充高度、进料速度、固定化酶用量、底物 摩尔比进行了研究。结果表明，固定化酶质量为47 g，填充高度40 cm(内径3．5 cm)，油醇摩尔比l：2．4，以4mL／min 速度进料保留时间4 h，产率可达83％。固定化酶在使用16批时，产率仍保持在70％以上。同时，对于填充床的 填充方式作了初步探索。 关键词：填充床反应器；蜡酯；脂肪酶 中图分类号：Q 814．3 文献标识码：A 文章编号：1000—6613(2009)05—0864—05 Synthesis of wax ester in an immobilized packed-bed reactor LlU Tingting，DENGLi，LUJiket TAN Tianwei，WANGFang (College of Life Science and Technology，Beijing Key Laboratory of Bioprocess，Biorefinery Research and Engineering Center，Ministry of Education，Beijing University of Chemical Technology，Beijing 100029，China) Abstract：WaX ester was synthesized from soybean oil and cetyl alcohol in a packed—bed reactor wim petroleum ether as solvent and immobilized lipase Candida sp．99—1 25 as catalyst．The effects of the height of packed．bed，flow rate of reactant，dosage of immobilized lipase，molar ratio of substrate were investigated．The optimum conditions were as follows：immobilized lipase 47g，the height of packed bed was 40cm rdiameter 3．5cm)，the molar ratio of oil to cetyl alcohol was l：2．4，the flow rate of reactant was 4mL／min and the retention time was 4h，the yield of WaX ester reached 83％ ．The stability of the immobilized lipase was also studied，and the yield remained above 70％after 1 6 batches．The pa