氮源影响酵母耐受超高浓度乙醇发酵胁迫条件.pdfVIP

学兔兔 化 工 进 展 2010年第29卷第 9期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ·1719· 氮源影响酵母耐受超高浓度乙醇发酵胁迫条件 秦 晴，胡纯铿，洪 美，高培培，顾中凤 (华侨大学化工学院，福建 厦门 361021) 摘 要：为考察蛋白胨和酵母浸出膏对酵母耐受超高浓度乙醇发酵胁迫条件的影响，以300g／L起始浓度葡萄糖 开展实验。结果表明，与对照组(3 g／L蛋白胨+5 g／L酵母浸出膏作为氮源)相比，单独提高发酵培养基蛋白胨 至6 g，L或酵母浸出膏至12 g／U，均可明显促进茵体生长和葡萄糖利用，终点乙醇体积分数由对照组的13．1％分 别提高至 14．4％和14．7％。研究表明，在发酵过程中，生长于提高蛋白胨浓度或酵母浸出膏浓度培养基的菌体， 其质膜ATP酶活力和胞内海藻糖积累量明显高于对照组，而且发酵参数 (如菌体生长、葡萄糖利用和终点乙醇 体积分数)的提高与酶活力和海藻糖含量的增加密切相关，提示质膜 ATP酶和胞内海藻糖在酵母耐受超高浓度 乙醇发酵胁迫条件中的作用。 关键词：超高浓度发酵；质膜ATP酶；海藻糖 中图分类号：Q 815 文献标志码：A 文章编号：1000—6613(2010)09—1719—06 Effect of nitrogen sources on stress tolerance of yeast in very high gravity ethanol fermentation QIN Qing，HU Chunkeng，HONG Mei，GAO Peipei，GU Zhongfeng (School ofChemical Engineering，Huaqiao University，Xiamen 361021，Fujian，China) Abstract：To investigate the impact of peptone or yeast extract on yeast stress tolerance during very high gravity(VHG)ethanol fermentation，experiments were conducted using 300 g／L initial glucose． Results showed that the increase of peptone from 3 to 6 g／L，or yeast extract from 5 to 12 g／L，in the ferm entation medium resulted in a noticeable increase in cell growth and glucose consumption compared with the control experiment using 3 g／L peptone plus 5 g／L yeast extract as nitrogen source． The final ethanol was increased from 13．1％ to 14．4％ and 14．7％ (v／v)respectively．During the ferm entation process，it was found that the cell growth in the increased level of peptone or yeast extract exhibited significantly higher plasma membran