Determination of Diniconazole in Agricultural Samples by Sol-Gel Immunoaffinity Extraction Procedure Coupled with HPLC and ELISA 英文参考文献.docVIP

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Determination of Diniconazole in Agricultural Samples by Sol-Gel Immunoaffinity Extraction Procedure Coupled with HPLC and ELISA 英文参考文献.doc

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Determination of Diniconazole in Agricultural Samples by Sol-Gel Immunoaffinity Extraction Procedure Coupled with HPLC and ELISA 英文参考文献

DeterminationofDiniconazoleinAgriculturalSamples bySol-GelImmunoaffinityExtractionProcedureCoupled withHPLCandELISA ZhenjiangLiu,YahuiJin,MinghuaWang* DepartmentofPesticideScience,CollegeofPlantProtection,NanjingAgriculturalUniversity,JiangsuKeyLaboratoryofPesticideScience,KeyLaboratoryofIntegrated ManagementofCropDiseasesandPests,MinistryofEducation,Nanjing,China Abstract Background: In the European Union (EU), the use of diniconazole-M is no longer authorized. However, residues of diniconazole-Moccurinvariousplantcommodities. Methodology/PrincipalFindings:Aselectiveandsimpleanalyticalmethodforthetraceleveldeterminationofdiniconazole insoil,fruit,vegetablesandwatersampleswasdevelopedbasedonimmunoaffinityextractionfollowedbyEnzyme-linked immunosorbentassay(ELISA)andthehigh-performanceliquidchromatography(HPLC)analysis.TheELISAwasbasedon monoclonalantibodieshighlyspecifictodiniconazoleandwasafast,cost-effective,andselectivescreeningmethodforthe detection of diniconazole. The results of the ELISA correlated well with gas chromatography (GC) results, with the correlationcoefficientof0.9879(n=19).Asimplegelpermeationchromato-graphyclean-upmethodwasdevelopedto purifyextractsfrommatricescontaininghighamountsoffatandnaturalpigments,withouttheneedforalargedilutionof thesample.Theimmunoaffinitycolumn(IAC)capacitywas0.180mgg21.Thecolumnscouldbere-usedapproximately20 timeswithnosignificantalterationincapacity.Therecoveriesfromcomplexsampleswereintherangeof89.2%to96.1% with a relative standard deviation (RSD) of 0.770%–6.11% by ELISA. The results were in good agreement with those obtainedbyHPLCmethod. Conclusion/Significance: The IAC extraction procedure coupled with HPLC and ELISA analysis could be also used as alternativeeffectiveanalyticalmethodsforthedeterminationofdiniconazoleconcentrationsincomplexsamples. Citation:LiuZ,JinY,WangM(2012)DeterminationofDiniconazoleinAgriculturalSamplesbySol-GelImmunoaffinityExtractionProcedureCoupledwithHPLC andELISA.PLoSONE7(10):e46929.d