Differences in the Pathways of Proteins Unfolding Induced by Urea and Guanidine Hydrochloride Molten Globule State and Aggregates 英文参考文献.docVIP

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Differences in the Pathways of Proteins Unfolding Induced by Urea and Guanidine Hydrochloride Molten Globule State and Aggregates 英文参考文献.doc

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Differences in the Pathways of Proteins Unfolding Induced by Urea and Guanidine Hydrochloride Molten Globule State and Aggregates 英文参考文献

DifferencesinthePathwaysofProteinsUnfolding InducedbyUreaandGuanidineHydrochloride:Molten GlobuleStateandAggregates OlgaI.Povarova,IrinaM.Kuznetsova,KonstantinK.Turoverov* LaboratoryofStructuralDynamics,StabilityandFoldingofProteins,InstituteofCytologyoftheRussianAcademyofScience,St.Petersburg,Russia Abstract Itwasshownthatatlowconcentrationsguanidinehydrochloride(GdnHCl)cancauseaggregationofproteinsinpartially foldedstateandthatfluorescentdye1-anilinonaphthalene-8-sulfonicacid(ANS)bindswiththeseaggregatesratherthan withhydrophobicclustersonthesurfaceofproteininmoltenglobulestate.ThatiswhytheincreaseinANSfluorescence intensity is often recorded in thepathway of protein denaturation by GdnHCl, but not by urea. So what was previously believedtobethemoltenglobulestateinthepathwayofproteindenaturationbyGdnHCl,inreality,forsomeproteins representstheaggregatesofpartiallyfoldedmolecules. Citation:PovarovaOI,KuznetsovaIM,TuroverovKK(2010)DifferencesinthePathwaysofProteinsUnfoldingInducedbyUreaandGuanidineHydrochloride: MoltenGlobuleStateandAggregates.PLoSONE5(11):e15035.doi:10.1371/journal.pone.0015035 Editor:VladimirN.Uversky,UniversityofSouthFloridaCollegeofMedicine,UnitedStatesofAmerica ReceivedSeptember3,2010;AcceptedOctober11,2010;PublishedNovember30,2010 Copyright: ? 2010 Povarova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbytheProgram‘‘MolecularandCellBiology’’oftheRussianAcademyofSciences.Thefunderhadnoroleinstudydesign, datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:kkt@mail.cytspb.rssi.ru Introduction ResultsandDiscussion Proteinunfoldinginducedbychemicaldenaturantssuchasurea andguanidinehydrochloride(GdnHCl)isacommonapproachto study protein foldin