Differential Expression of miRNAs in Response to Topping in Flue-Cured Tobacco (Nicotiana tabacum) Roots 英文参考文献.docVIP

下载本文档

14
0
约6.15万字
约 15页
2017-05-11 发布于上海
举报
版权申诉

Differential Expression of miRNAs in Response to Topping in Flue-Cured Tobacco (Nicotiana tabacum) Roots 英文参考文献.doc

1、本文档共15页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Differential Expression of miRNAs in Response to Topping in Flue-Cured Tobacco (Nicotiana tabacum) Roots 英文参考文献

DifferentialExpressionofmiRNAsinResponseto ToppinginFlue-CuredTobacco(Nicotianatabacum) Roots HongxiangGuo1.,YunchaoKan2.,WeiqunLiu1* 1The Key Lab of National Tobacco Cultivation, College of Life Sciences, Henan Agricultural University, Zhengzhou, China, 2China-UK NYNU-RRes Joint Lab of Insect Biology,NanyangNormalUniversity,Nanyang,China Abstract Background:Toppingisanimportantcultivatingmeasureforflue-curedtobacco,andmanygeneshadbeenfoundtobe differentiallyexpressedinresponsetotopping.Butitisstillunclearhowthesegenesareregulated.MiRNAsplayacritical role in post-transcriptional gene regulation, so we sequenced two sRNA libraries from tobacco roots before and after topping,withaviewtoexploringtranscriptionaldifferencesinmiRNAs. Methodology/PrincipalFindings:TwosRNAlibrariesweregeneratedfromtobaccorootsbeforeandaftertopping.Solexa high-throughput sequencing of tobacco small RNAs revealed a total of 12,104,207 and 11,292,018 reads representing 3,633,398and3,084,102distinctsequencesbeforeandaftertopping.Theexpressionsof136conservedmiRNAs(belonging to32families)and126newmiRNAs(belongingto77families)weredetermined.TherewerethreemajorconservedmiRNAs families(nta-miR156,nta-miR172andnta-miR171)andtwomajornewmiRNAsfamilies(nta-miRn2andnta-miRn26).Allof theseidentifiedmiRNAscanbefoldedintocharacteristicmiRNAstem-loopsecondaryhairpinstructures,andqRT-PCRwas adoptedtovalidateandmeasuretheexpressionofmiRNAs.Putativetargetswereidentifiedfor133outof136conserved miRNAsand126newmiRNAs.OfthesemiRNAswhosetargetshadbeenidentified,themiRNAswhichchangemarkedly(.2 folds)belongto53familiesandtheirtargetshavedifferentbiologicalfunctionsincludingdevelopment,responsetostress, responsetohormone,Nmetabolism,Cmetabolism,signaltransduction,nucleicacidmetabolismandothermetabolism. SomeinterestingtargetsformiRNAshadbeendetermined. Conclusions/Significance: The differential expression profiles of miRNAs were shown in flue-cured tobacco roots before and after topping, which can be expected to regu