DNA Adenine Methylation Is Required to Replicate Both Vibrio cholerae Chromosomes Once per Cell Cycle 英文参考文献.docVIP

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DNA Adenine Methylation Is Required to Replicate Both Vibrio cholerae Chromosomes Once per Cell Cycle 英文参考文献.doc

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DNA Adenine Methylation Is Required to Replicate Both Vibrio cholerae Chromosomes Once per Cell Cycle 英文参考文献

DNAAdenineMethylationIsRequiredtoReplicateBoth VibriocholeraeChromosomesOnceperCellCycle Gae¨lleDemarre¤,DhrubaK.Chattoraj* CenterforCancerResearch,NationalCancerInstitute,Bethesda,Maryland,UnitedStatesofAmerica Abstract DNA adenine methylation is widely used to control many DNA transactions, including replication. In Escherichia coli, methylation serves to silence newly synthesized (hemimethylated) sister origins. SeqA, a protein that binds to hemimethylated DNA, mediates the silencing, and this is necessary to restrict replication to once per cell cycle. The methylation,however,isnotessentialforreplicationinitiationpersebutappearedsowhentheorigins(oriIandoriII)ofthe twoVibriocholeraechromosomeswereusedtodriveplasmidreplicationinE.coli.Hereweshowthat,asinthecaseofE. coli, methylation is not essential for oriI when it drives chromosomal replication and is needed for once-per-cell-cycle replication in a SeqA-dependent fashion. We found that oriII also needs SeqA for once-per-cell-cycle replication and, additionally, full methylation for efficient initiator binding. The requirement for initiator binding might suffice to make methylationanessentialfunctioninV.cholerae.ThestructureoforiIIsuggeststhatitoriginatedfromaplasmid,butunlike plasmids, oriII makes use of methylation for once-per-cell-cycle replication, the norm for chromosomal but not plasmid replication. Citation: Demarre G, Chattoraj DK (2010) DNA Adenine Methylation Is Required to Replicate Both Vibrio cholerae Chromosomes Once per Cell Cycle. PLoS Genet6(5):e1000939.doi:10.1371/journal.pgen.1000939 Editor:WilliamF.Burkholder,StanfordUniversity,UnitedStatesofAmerica ReceivedJune26,2009;AcceptedApril6,2010;PublishedMay6,2010 Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsPublicDomaindeclarationwhichstipulatesthat,onceplacedinthepublic domain,thisworkmaybefreelyreproduced,distributed,transmitted,modified,builtupon,orotherwiseusedbyanyoneforanylawfulpurpose. Funding:Thisworkwassupporte