Down-Regulation of AP-4 Inhibits Proliferation, Induces Cell Cycle Arrest and Promotes Apoptosis in Human Gastric Cancer Cells 英文参考文献.docVIP

下载本文档

8
0
约 10页
2017-05-11 发布于上海
举报
版权申诉

Down-Regulation of AP-4 Inhibits Proliferation, Induces Cell Cycle Arrest and Promotes Apoptosis in Human Gastric Cancer Cells 英文参考文献.doc

1、本文档共10页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Down-Regulation of AP-4 Inhibits Proliferation, Induces Cell Cycle Arrest and Promotes Apoptosis in Human Gastric Cancer Cells 英文参考文献

Down-RegulationofAP-4InhibitsProliferation,Induces CellCycleArrestandPromotesApoptosisinHuman GastricCancerCells XinghuaLiu1.,BoZhang1.,YanGuo2,QiLiang1,ChangyaoWu1,LeiWu1,KaixiongTao1, GuobinWang1*,JianyingChen1* 1DepartmentofGeneralSurgery,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan,China,2DepartmentofMaternaland ChildHealth,SchoolofPublicHealth,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan,China Abstract Background: AP-4 belongs to the basic helix-loop-helix leucine-zipper subgroup; it controls target gene expression, regulates growth, development and cell apoptosis and has been implicated in tumorigenesis. Our previous studies indicatedthatAP-4wasfrequentlyoverexpressedingastriccancersandmaybeassociatedwiththepoorprognosis.The purposeofthisstudyistoexaminewhethersilencingofAP-4canalterbiologicalcharacteristicsofgastriccancercells. Methods:TwospecificsiRNAstargetingAP-4weredesigned,synthesized,andtransfectedintogastriccancercelllinesand human normal mucosa cells. AP-4 expression was measured with real-time quantitative PCR and Western blot. Cell proliferationandchemo-sensitivityweredetectedbyCCK-8assay.Cellcycleassayandapoptosisassaywereperformedby flowcytometer,andrelativeexpressionofcellcycleregulatorsweredetectedbyreal-timequantitativePCRandWestern blot,expressionofthefactorsinvolvedintheapoptosispathwaywereexaminedinmRNAandproteinlevel. Results:TheexpressionofAP-4wassilencedbythesiRNAstransfectionandtheeffectsofAP-4knockdownlasted24to 96hrs.ThesiRNA-mediatedsilencingofAP-4suppressedthecellularproliferation,inducedapoptosisandsensitizedcancer cells to anticancer drugs. In addition, the expression level of p21, p53 and Caspase-9 were increased when AP-4 was knockdown,buttheexpressionofcyclinD1,Bcl-2andBcl-xLwasinhibited.Itdidn’tinducecellcyclearrestwhenAP-4was knockdowninp53defectgastriccancercelllineKato-III. Conclusions: These results illustrated that gene silencing of AP-4 can efficiently inhibited