Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour 英文参考文献.docVIP

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Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour 英文参考文献.doc

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Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour 英文参考文献

DrosophilaRbp6IsanOrthologueofVertebrateMsi-1 andMsi-2,butDoesNotFunctionRedundantlywith dMsitoRegulateGermlineStemCellBehaviour NicoleA.Siddall1,2,MarinaKalcina1,2,TimothyM.Johanson1,AdrianC.Monk1,2,FrancaCasagranda1, ReevaP.Been1,EileenA.McLaughlin2,3,GaryR.Hime1,2 * 1AnatomyandNeuroscience,UniversityofMelbourne,Parkville,Australia,2ARCCentreofExcellenceinBiotechnologyandDevelopment,Callaghan,Australia,3School ofEnvironmentalandLifeScience,UniversityofNewcastle,Callaghan,Australia Abstract The vertebrate RNA-binding proteins, Musashi-1 (Msi-1) and Musashi-2 (Msi-2) are expressed in multiple stem cell populations.AroleforMusashiproteinsinpreventingstemcelldifferentiationhasbeensuggestedfromgeneticanalysisof theDrosophilafamilymember,dMsi,andbothvertebrateMsiproteinsfunctionco-operativelytoregulateneuralstemcell behaviour.HerewehaveidentifiedasecondDrosophilaMsifamilymember,Rbp6,whichsharesmoreaminoacididentity withvertebrateMsi-1andMsi-2thandMsi.WegeneratedanantibodythatdetectsmostRbp6spliceisoformsandshowthat Rbp6isexpressedinmultipletissuesthroughoutdevelopment.However,Rbp6deletionmutantsgeneratedinthisstudyare viableandfertile,andshowonlyminordefects.WeusedDrosophilaspermatogonialgermlinestemcells(GSC’s)asamodel totestwhetherDrosophilaMsiproteinsfunctionredundantlytoregulatestemcellbehaviour.However,likevertebrateMsi- 1andMsi-2,Rbp6andMsidonotappeartobeco-expressedinspermatogenicGSC’sanddonotfunctionco-operativelyin theregulationofGSCmaintenance.ThuswhiletwoMsifamilymembersarepresentinDrosophila,thefunctionofthefamily membershavediverged. Citation:SiddallNA,KalcinaM,JohansonTM,MonkAC,CasagrandaF,etal.(2012)DrosophilaRbp6IsanOrthologueofVertebrateMsi-1andMsi-2,butDoesNot FunctionRedundantlywithdMsitoRegulateGermlineStemCellBehaviour.PLoSONE7(11):e49810.doi:10.1371/journal.pone.0049810 Editor:BarbaraJennings,UniversityCollegeLondon,UnitedKingdom ReceivedJune18,2012;AcceptedOctober17,2012;PublishedNovember27,2012 Copyright: ? 2012 Siddall etal. This is an open-ac