Drosophila tan Encodes a Novel Hydrolase Required in Pigmentation and Vision 英文参考文献.docVIP

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Drosophila tan Encodes a Novel Hydrolase Required in Pigmentation and Vision 英文参考文献.doc

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Drosophila tan Encodes a Novel Hydrolase Required in Pigmentation and Vision 英文参考文献

DrosophilatanEncodesaNovelHydrolase RequiredinPigmentationandVision John R.True1*,Shu-Dan Yeh1,Bernhard T.Hovemann2,Tobias Kemme2,Ian A.Meinertzhagen3,Tara N.Edwards4, Shian-Ren Liou1,Qian Han5,Jianyong Li5 1DepartmentofEcologyandEvolution,StateUniversityofNewYork,StonyBrook,NewYork,UnitedStatesofAmerica,2Fakulta¨t fu¨rChemie,Ruhr-Universita¨t Bochum, Bochum,Germany,3LifeSciencesCentre,DalhousieUniversity,Halifax,NovaScotia,Canada,4DepartmentofBiology,DalhousieUniversity,Halifax,NovaScotia,Canada, 5DepartmentofVeterinaryPathology,CollegeofVeterinaryMedicine,UniversityofIllinois,Urbana,Illinois,UnitedStatesofAmerica Manyproteinsareusedrepeatedlyindevelopment,butusuallythefunctionoftheproteinissimilarinthedifferent contexts.HerewereportthattheclassicalDrosophilamelanogasterlocustanencodesanovelenzymerequiredfortwo verydifferentcellularfunctions:hydrolysisofN-b-alanyldopamine(NBAD)todopamineduringcuticularmelanization, andhydrolysisofcarcininetohistamineinthemetabolismofphotoreceptorneurotransmitter.Wecharacterizedtwo tan-likeP-elementinsertionsthatfailedtocomplementclassicaltanmutations.Bothareinsertedinthe59untranslated regionofthepreviouslyuncharacterizedgeneCG12120,aputativehomologoffungalisopenicillin-NN-acyltransferase (EC 64). Both P insertions showed abnormally low transcription of the CG12120 mRNA. Ectopic CG12120 expression rescued tan mutant pigmentation phenotypes and caused the production of striking black melanin patterns.ElectroretinogramandheadhistamineassaysindicatedthatCG12120isrequiredforhydrolysisofcarcinine to histamine, which is required for histaminergic neurotransmission. Recombinant CG12120 protein efficiently hydrolyzed both NBAD to dopamine and carcinine to histamine. We conclude that D. melanogaster CG12120 corresponds to tan. This is, to our knowledge, the first molecular genetic characterization of NBAD hydrolase and carcininehydrolaseactivityinanyorganismandiscentraltotheunderstandingofpigmentationandphotoreceptor functio