Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation 英文参考文献.docVIP

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Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation 英文参考文献.doc

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Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation 英文参考文献

EffectofCigaretteSmokeExtractonDendriticCellsand TheirImpactonT-CellProliferation EsmaeilMortaz1,2,3*,AlettaD.Kraneveld1,JoostJ.Smit4,MirjamKool5,6,BartN.Lambrecht5,6,StevenL. Kunkel7,NicholasW.Lukacs7,FransP.Nijkamp1,GertFolkerts1 1Division of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands, 2Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran, 3Department of Basic Science, Section of Biochemistry, Faculty of Veterinary Medicine,UrmiaUniversity,Urmia,Iran,4InstituteforRiskAssessmentSciences(IRAS),UtrechtUniversity,Utrecht,TheNetherlands,5DepartmentofPulmonaryMedicine, ErasmusUniversityMedicalCenter,Rotterdam,TheNetherlands,6LaboratoryofImmunoregulationandMucosalImmunity,DepartmentofPulmonaryMedicine,Ghent University,Ghent,Belgium,7DepartmentofPathology,UniversityofMichiganMedicalSchool,AnnArbor,Michigan,UnitedStatesofAmerica Abstract Chronicobstructivepulmonarydisease(COPD)ischaracterizedbychronicairwayinflammation.Cigarettesmokehasbeen considered a major player in the pathogenesis of COPD. The inflamed airways of COPD patients contain several inflammatorycellsincludingneutrophils,macrophages,Tlymphocytes,anddendriticcells(DCs).Therelativecontributions ofthesevariousinflammatorycellstoairwayinjuryandremodelingarenotwelldocumented.Inparticular,thepotential roleofDCsasmediatorsofinflammationinthesmoker’sairwaysandCOPDpatientsispoorlyunderstood.Inthecurrent study we analyzed the effects of cigarette smoke extract on mouse bone marrow derived DC and the production of chemokines and cytokines were studied. In addition, we assessed CSE-induced changes in cDC function in the mixed lymphocytereaction(MLR)examiningCD4+andCD8+Tcellproliferation.Cigarettesmokeextractinducesthereleaseof the chemokines CCL3 and CXCL2 (but not cytokines), via the generation of reactive oxygen species (ROS). In a mixed- + leukocytereactionassay,cigarettesmoke-primedDCspot