Ecological Niche Modelling and nDNA Sequencing Support a New, Morphologically Cryptic Beetle Species Unveiled by DNA Barcoding 英文参考文献.docVIP
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Ecological Niche Modelling and nDNA Sequencing Support a New, Morphologically Cryptic Beetle Species Unveiled by DNA Barcoding 英文参考文献
EcologicalNicheModellingandnDNASequencing
SupportaNew,MorphologicallyCrypticBeetleSpecies
UnveiledbyDNABarcoding
OliverHawlitschek1,NickPorch2,LarsHendrich1,MichaelBalke1,3*
1Department of Entomology, Zoological State Collection, Munich, Germany, 2School of Life and Environmental Sciences, Deakin University, Melbourne, Australia,
3GeoBioCenter,Ludwig-Maximilians-University,Munich,Germany
Abstract
Background:DNAsequencingtechniquesusedtoestimatebiodiversity,suchasDNAbarcoding,mayrevealcrypticspecies.
However,disagreementsbetweenbarcodingandmorphologicaldatahavealreadyledtocontroversy.Speciesdelimitation
shouldtherefore notbe basedonmtDNA alone.Here,weexploretheuseofnDNAandbioclimatic modellinginanew
speciesofaquaticbeetlerevealedbymtDNAsequencedata.
Methodology/Principal Findings: The aquatic beetle fauna of Australia is characterised by high degrees of endemism,
includinglocalradiationssuchasthegenusAntiporus.Antiporusfemoraliswaspreviouslyconsideredtoexistintwodisjunct,
but morphologically indistinguishable populations in south-western and south-eastern Australia. We constructed a
phylogeny of Antiporus and detected a deep split between these populations. Diagnostic characters from the highly
variablenuclearproteinencodingargininekinasegeneconfirmedthepresenceoftwoisolatedpopulations.Wethenused
ecological niche modelling to examine the climatic niche characteristics of the two populations. All results support the
statusofthetwopopulationsasdistinctspecies.Wedescribethesouth-westernspeciesasAntiporusoccidentalissp.n.
Conclusion/Significance: In addition to nDNA sequence data and extended use of mitochondrial sequences, ecological
nichemodellinghasgreatpotentialfordelineatingmorphologicallycrypticspecies.
Citation:HawlitschekO,PorchN,HendrichL,BalkeM(2011)EcologicalNicheModellingandnDNASequencingSupportaNew,MorphologicallyCrypticBeetle
SpeciesUnveiledbyDNABarcoding.PLoSONE6(2):e16662.doi:10.1371/journal.pone.0016662
Editor:DavidNogues-Bravo,UniversityofCopenh
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