Evidence for Regulated Interleukin-4 Expression in Chondrocyte-Scaffolds under In Vitro Inflammatory Conditions 英文参考文献.docVIP

下载本文档

4
0
约4.84万字
约 10页
2017-05-11 发布于上海
举报
版权申诉

Evidence for Regulated Interleukin-4 Expression in Chondrocyte-Scaffolds under In Vitro Inflammatory Conditions 英文参考文献.doc

1、本文档共10页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Evidence for Regulated Interleukin-4 Expression in Chondrocyte-Scaffolds under In Vitro Inflammatory Conditions 英文参考文献

EvidenceforRegulatedInterleukin-4Expressionin Chondrocyte-ScaffoldsunderInVitroInflammatory Conditions MuhammadFarooqRai1¤,ThomasGraeve1,SvenTwardziok3,MichaelF.G.Schmidt1* 1InstituteofImmunologyandMolecularBiology,DepartmentofVeterinaryMedicine,FreieUniversita¨t Berlin,Berlin,Germany,2AmedrixGmbH,Esslingen,Germany, 3InstituteofMolecularBiologyandBioinformatics,ChariteUniversityofMedicine,BenjaminFranklinCampus,Berlin,Germany Abstract Objective:Toelucidatetheanti-inflammatoryandanaboliceffectsofregulatedexpressionofIL-4inchondrocyte-scaffolds underinvitroinflammatoryconditions. Methods: Mature articular chondrocytes from dogs (n=3) were conditioned through transient transfection using pcDNA3.1.cIL-4 (constitutive) or pCOX-2.cIL-4 (cytokine-responsive) plasmids. Conditioned cells were seeded in alginate microspheres and rat-tail collagen type I matrix (CaReSH) to generate two types of tissue-engineered 3-dimensional scaffolds. Inflammatory arthritis was simulated in the packed chondrocytes through exogenous addition of recombinant canine(rc)IL-1b(100ng/ml)plusrcTNFa(50ng/ml)inculturemediafor96hours.Harvestedcellsandculturemediawere analyzedbyvariousassaystomonitortheanti-inflammatoryandregenerative(anabolic)propertiesofcIL-4. Results:cIL-4wasexpressedfromCOX-2promoterexclusivelyontheadditionofrcIL-1bandrcTNFawhileitsexpression fromCMVpromoterwasconstitutive.TheexpressedcIL-4downregulatedthemRNAexpressionofIL-1b,TNFa,IL-6,iNOS andCOX-2inthecellsandinhibitedtheproductionofNOandPGE2inculturemedia.Atthesametime,itup-regulatedthe expressionofIGF-1,IL-1ra,COL2a1andaggrecaninconditionedchondrocytesinbothscaffoldsalongwithadiminished release of total collagen and sGAG into the culture media. An increased amount of cIL-4 protein was detected both in chondrocytecelllysateandinconcentratedculturemedia.Neutralizinganti-cIL-4antibodyassayconfirmedthattheanti- inflammatoryandregenerativeeffectsseenareexclusivelydrivenbycIL-4.TherewasarestrictedexpressionofIL-4under COX-2p