Expression of HA of HPAI H5N1 Virus at US2 Gene Insertion Site of Turkey Herpesvirus Induced Better Protection than That at US10 Gene Insertion Site 英文参考文献.docVIP

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Expression of HA of HPAI H5N1 Virus at US2 Gene Insertion Site of Turkey Herpesvirus Induced Better Protection than That at US10 Gene Insertion Site 英文参考文献.doc

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Expression of HA of HPAI H5N1 Virus at US2 Gene Insertion Site of Turkey Herpesvirus Induced Better Protection than That at US10 Gene Insertion Site 英文参考文献

ExpressionofHAofHPAIH5N1VirusatUS2Gene InsertionSiteofTurkeyHerpesvirusInducedBetter ProtectionthanThatatUS10GeneInsertionSite HongboGao1.,HongyuCui1,2.,XianlanCui3,XingmingShi1,YanZhao1,XiaoyanZhao1 ,Yanming Quan1,ShuaiYan1,WeiweiZeng1,YunfengWang1* 1Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences,Harbin,China,2DepartmentofAnimalMedicine,CollegeofAnimalScienceandVeterinaryMedicine,HebeiNorthUniversity,Zhang-Jia-Kou,China,3Animal HealthLaboratory,DepartmentofPrimaryIndustries,Parks,WaterandEnvironment,Prospect,Australia Abstract Herpesvirusofturkey(HVT)isbeingwidelyusedasavectorfordevelopmentofrecombinantvaccinesandUS2andUS10 genesareoftenchosenasinsertionsitesfortargetedgeneexpression.However,thedifferenteffectsofthetwogenesfor generationofrecombinantHVTvaccineswereunknown.Inordertocomparetheeffectsofinsertedgenesinthetwosites on the efficacy of the recombinant vaccines, host-protective haemagglutinin (HA) gene of the highly pathogenic avian influenzavirus(HPAIV)H5N1wasinsertedintoeitherUS2orUS10genelocusoftheHVT.TheresultingUS2(rHVT-US2-HA) orUS10(rHVT-US10-HA)recombinantHVTviruseswereusedtoinfectchickenembryofibroblasts.Plaquesandthegrowth kineticsofrHVT-US2-HA-infectedchickenembryofibroblastsweresimilartothoseofparentalHVTwhereasrHVT-US10-HA infectedchickenembryofibroblastshaddifferentgrowthkineticsandplaqueformation.TheviremialevelsinrHVT-US10-HA virus-infectedchickensweresignificantlylowerthanthoseofrHVT-US2-HAgroupon28dayspostinfection.Thevaccine efficacyofthetworecombinantvirusesagainstH5N1HPAIVandvirulentMarek’sdiseaseviruswasalsoevaluatedin1-day- oldvaccinatedchickens.rHVT-US2-HA-vaccinatedchickenswerebetterprotectedwithreducedmortalitythanrHVT-US10- HA-vaccinated animals following HPAIV challenge. Furthermore, theoverall hemaglutination inhibition antibody titers of rHVT-US2-HA-vaccinatedchickenswerehigherthanthoseofrHVT-US10-HA-vacc