Expression of Novel Opsins and Intrinsic Light Responses in the Mammalian Retinal Ganglion Cell Line RGC-5. Presence of OPN5 in the Rat Retina 英文参考文献.docVIP

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Expression of Novel Opsins and Intrinsic Light Responses in the Mammalian Retinal Ganglion Cell Line RGC-5. Presence of OPN5 in the Rat Retina 英文参考文献.doc

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Expression of Novel Opsins and Intrinsic Light Responses in the Mammalian Retinal Ganglion Cell Line RGC-5. Presence of OPN5 in the Rat Retina 英文参考文献

ExpressionofNovelOpsinsandIntrinsicLightResponses intheMammalianRetinalGanglionCellLineRGC-5. PresenceofOPN5intheRatRetina PaulaS.Nieto,DiegoJ.Valdez,VictoriaA.Acosta-Rodr?′guez,MarioE.Guido* CIQUIBIC(CONICET)-DepartamentodeQu?′micaBiolo′gica,FacultaddeCienciasQu?′micas,UniversidadNacionaldeCo′rdoba,Co′rdoba,Argentina Abstract Thevertebrateretinaisknowntocontainthreeclassesofphotoreceptorcells:conesandrodsresponsibleforvision,and intrinsically photoresponsive retinal ganglion cells (RGCs) involved in diverse non-visual functions such as photic entrainment of daily rhythms and pupillary light responses. In this paper we investigated the potential intrinsic photoresponsivenessoftheratRGCline,RGC-5,bytestingforthepresenceofvisualandnon-visualopsinsandassessing 2+ expressionoftheimmediate-earlygeneproteinc-FosandchangesinintracellularCa mobilizationinresponsetobrieflight pulses.CulturedRGC-5cellsexpressanumberofphotopigmentmRNAssuchasretinalGproteincoupledreceptor(RGR), encephalopsin/panopsin (Opn3), neuropsin (Opn5) and cone opsin (Opn1mw) but not melanopsin (Opn4) or rhodopsin. Opn5immunoreactivitywasobservedinRGC-5cellsandintheinnerretinaofrat,mainlylocalizedintheganglioncelllayer (GCL).Furthermore,whitelightpulsesofdifferentintensitiesanddurationselicitedchangesbothinintracellularCa2+ levels andintheinductionofc-Fos proteininRGC-5cellcultures. TheresultsdemonstratethatRGC-5cells expressingdiverse putative functional photopigments display intrinsic photosensitivity which accounts for the photic induction of c-Fos protein and changes in intracellular Ca mobilization. The presence of Opn5 in the GCL of the rat retina suggests the 2+ existenceofanoveltypeofphotoreceptorcell. Citation: Nieto PS, Valdez DJ, Acosta-Rodr?′guez VA, Guido ME (2011) Expression of Novel Opsins and Intrinsic Light Responses in the Mammalian Retinal GanglionCellLineRGC-5.PresenceofOPN5intheRatRetina.PLoSONE6(10):e26417.doi:10.1371/journal.pone.0026417 Editor:StuartE.Dryer,Universit