Fluorescent Nanocrystals Reveal Regulated Portals of Entry into and Between the Cells of Hydra 英文参考文献.docVIP

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Fluorescent Nanocrystals Reveal Regulated Portals of Entry into and Between the Cells of Hydra 英文参考文献.doc

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Fluorescent Nanocrystals Reveal Regulated Portals of Entry into and Between the Cells of Hydra 英文参考文献

FluorescentNanocrystalsRevealRegulatedPortalsof EntryintoandBetweentheCellsofHydra ClaudiaTortiglione1*,AlessandraQuarta2,MariaAdaMalvindi1,3,AngelaTino1,TeresaPellegrino2,3 1Istituto di Cibernetica ‘‘E Caianiello’’, Consiglio Nazionale delle Ricerche (CNR), Pozzuoli, Italy, 2Fondazione Istituto Italiano di Tecnologia, Genova, Italy, 3National NanotechnologyLaboratoryofCNR-INFM,Unita` diricercaIITandScuolaSuperioreISUFI,Lecce,Italy Abstract Initiallyviewedasinnovativecarriersforbiomedicalapplications,withuniquephotophysicalpropertiesandgreatversatility tobe decorated attheirsurface with suitablemolecules,nanoparticles canalso play activeroles inmediatingbiological effects,suggestingtheneedtodeeplyinvestigatethemechanismsunderlyingcell-nanoparticleinteractionandtoidentify themolecularplayers.HereweshowthatthecelluptakeoffluorescentCdSe/CdSquantumrods(QRs)byHydravulgaris ,a simple model organism at the base of metazoan evolution, can be tuned by modifying nanoparticle surface charge. At acidic pH, amino-PEG coated QRs, showing positive surface charge, are actively internalized by tentacle and body ectodermal cells, while negatively charged nanoparticles are not uptaken. In order to identify the molecular factors underlyingQRuptakeatacidicpH,weprovidefunctionalevidenceofannexinsinvolvementandexplaintheQRuptakeas thecombinedresultofQRpositivechargeandannexinmembraneinsertion.Moreover,trackingQRlabelledcellsduring development and regeneration allowed us to uncover novel intercellular trafficking and cell dynamics underlying the remarkableplasticityofthisancientorganism. Citation:TortiglioneC,QuartaA,MalvindiMA,TinoA,PellegrinoT(2009)FluorescentNanocrystalsRevealRegulatedPortalsofEntryintoandBetweentheCells ofHydra.PLoSONE4(11):e7698.doi:10.1371/journal.pone.0007698 Editor:NeerajVij,JohnsHopkinsSchoolofMedicine,UnitedStatesofAmerica ReceivedJune15,2009;AcceptedOctober12,2009;PublishedNovember2,2009 Copyright: ?2009Tortiglioneetal.This isanopen-accessarticledistribu