FRAP Analysis Accounting for Bleaching during Image Capture 英文参考文献.docVIP

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FRAP Analysis Accounting for Bleaching during Image Capture 英文参考文献.doc

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FRAP Analysis Accounting for Bleaching during Image Capture 英文参考文献

FRAPAnalysis:AccountingforBleachingduringImage Capture JunWu1,NandiniShekhar1,PushkarP.Lele2,TanmayP.Lele1* 1Department of Chemical Engineering, University of Florida, Gainesville Florida, United States of America, 2Department of Molecular and Cellular Biology, Harvard University,Cambridge,Massachusetts,UnitedStatesofAmerica Abstract The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescencerecoveryprocess.AnimportantfeatureofFRAPexperimentsthattendstobeignoredinthemodelingisthat therecanbeasignificantlossoffluorescenceduetobleachingduringimagecapture.Inthispaper,weexplicitlyincludethe effectsofbleachingduringimage captureinthemodelfortherecoveryprocess, insteadofcorrecting fortheeffects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approachinFRAPanalysis. Citation: Wu J, Shekhar N, Lele PP, Lele TP (2012) FRAP Analysis: Accounting for Bleaching during Image Capture. PLoS ONE 7(8): e42854. doi:10.1371/ journal.pone.0042854 Editor:MichaelA.Mancini,BaylorCollegeofMedicine,UnitedStatesofAmerica ReceivedApril10,2012;AcceptedJuly12,2012;PublishedAugust9,2012 Copyright:?2012Wuetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was supported by the National Science Foundation under awards CMMI 0954302 (TPL) and CMMI 0927945 (TPL). / awards/award_visualization_noscript.jsp?org=CMMIHion=US-FLinstId=0015354000. The funders had no role in study design, data collection and analysis, decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:tlele@ Introduction applicable for many situations, it is obvious that if there was significantbleachingasaresultoftheimagecaptureprocessitself, thenF(h)v