Full Sequence and Comparative Analysis of the Plasmid pAPEC-1 of Avian Pathogenic E. coli χ7122 (O78∶K80∶H9) 英文参考文献.docVIP

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Full Sequence and Comparative Analysis of the Plasmid pAPEC-1 of Avian Pathogenic E. coli χ7122 (O78∶K80∶H9) 英文参考文献.doc

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Full Sequence and Comparative Analysis of the Plasmid pAPEC-1 of Avian Pathogenic E. coli χ7122 (O78∶K80∶H9) 英文参考文献

FullSequenceandComparativeAnalysisofthePlasmid pAPEC-1ofAvianPathogenicE.colix7122(O78:K80:H9) MelhaMellata1,JeffreyW.Touchman2,RoyCurtissIII1,2* 1TheBiodesignInstitute,ArizonaStateUniversity,Tempe,Arizona,UnitedStatesofAmerica,2SchoolofLifeSciences,ArizonaStateUniversity,Tempe,Arizona,United StatesofAmerica Abstract Background: Extra-intestinal pathogenic E. coli (ExPEC), including Avian Pathogenic E. coli (APEC), are very diverse. They cause a complex of diseases in Human, animals, and birds. Even though large plasmids are often associated with the virulenceofExPEC,theircharacterizationisstillinitsinfancy. Methodology/Principal Findings: We fully sequenced and analyzed the large plasmid pAPEC-1 (103,275-bp) associated withtheAPECstrainx7122,fromworldwideserogroupO78:K80:H9.Aputativevirulenceregionspanningan80-kbregion of pAPEC-1 possesses four iron acquisition systems (iutA iucABCD, sitABCD, iroBCDN, and temperature-sensitive hemagglutinin tsh), a colicin V operon, increasing serum sensitivity iss, ompT, hlyF, and etsABC. Thirty three ORFs in pAPEC-1areidentifiedasinsertionsequences(ISs)thatbelongtoninefamilieswithdiverseorigins.Thefulllengthofthe transferregion inpAPEC-1(11kb)isshorter comparedtothe tra regionofothersequencedFplasmids;theabsenceof sometragenesinpAPEC-1affectsitsself-transferability,andtheconjugativefunctionoftheplasmidwaseffectiveonlyin thepresenceofotherplasmids.Two-repliconsystems,repFIIA-repFICandrepFIB,andtwopost-segregationalsystems,srnB andhok/sok,arealsopresentinthesequenceofpAPEC-1.ThecomparisonofthepAPEC-1sequencewiththetwoavailable plasmid sequences reveals more gene loss and reorganization than previously appreciated. The presence of pAPEC-1- associatedgenesisassessedinhumanExPECbyPCR.Manypatternsofassociationbetweengenesarefound. Conclusions/Significance: The pathotype typical of pAPEC-1 was present in some human strains, which indicates a horizontal transfer between strains and the zoonotic risk of APEC strains. ColV plasmids