Function of SSA Subfamily of Hsp70 Within and Across Species Varies Widely in Complementing Saccharomyces cerevisiae Cell Growth and Prion Propagation 英文参考文献.docVIP

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Function of SSA Subfamily of Hsp70 Within and Across Species Varies Widely in Complementing Saccharomyces cerevisiae Cell Growth and Prion Propagation 英文参考文献.doc

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Function of SSA Subfamily of Hsp70 Within and Across Species Varies Widely in Complementing Saccharomyces cerevisiae Cell Growth and Prion Propagation 英文参考文献

FunctionofSSASubfamilyofHsp70WithinandAcross SpeciesVariesWidelyinComplementingSaccharomyces cerevisiaeCellGrowthandPrionPropagation DeepakSharma1.,Ce′lineN.Martineau2.,Marie-The′re`seLeDall3,MichaelReidy1,DanielC.Masison1 * , MehdiKabani2* 1LaboratoryofBiochemistryandGenetics,NationalInstitutesofDiabetes,DigestiveandKidneyDiseases,NationalInstitutesofHealth,Bethesda,Maryland,UnitedStates ofAmerica,2Laboratoired’EnzymologieetBiochimieStructurales,CentreNationaldelaRechercheScientifique,Gif-sur-Yvette,France,3LaboratoiredeMicrobiologieet Ge′ne′tiqueMole′culaire,CentreNationaldelaRechercheScientifique,InstitutNationaldelaRechercheAgronomique,AgroParisTech,Thiverval-Grignon,France Abstract Background:ThecytosolofmosteukaryoticcellscontainsmultiplehighlyconservedHsp70orthologsthatdiffermainlyby theirspatio-temporalexpressionpatterns.Hsp70splayessentialrolesinproteinfolding,transportordegradation,andare major players of cellular quality control processes. However, while several reports suggest that specialized functions of Hsp70orthologswereselectedthroughevolution,fewstudiesaddressedsystematicallythisissue. Methodology/PrincipalFindings:WecomparedtheabilityofSsa1p-Ssa4pfromSaccharomycescerevisiaeandSsa5p-Ssa8p from the evolutionary distant yeast Yarrowia lipolytica to perform Hsp70-dependent tasks when expressed as the sole Hsp70forS.cerevisiaeinvivo.WeshowthatHsp70isoforms(i)supportedyeastviabilityyetwithmarkedlydifferentgrowth + rates, (ii) influenced the propagation and stability of the [PSI ] and [URE3] prions, but iii) did not significantly affect the proteasomaldegradationrateofCFTR.Additionally,weshowthatindividualHsp70orthologsdidnotinducetheformation ofdifferentprionstrains,butratherinfluencedtheaggregationpropertiesofSup35invivo.Finally,weshowthat[URE3] curingbytheoverexpressionofYdj1pisHsp70-isoformdependent. Conclusion/Significance: Despite very high homology and overlapping functions, the different Hsp70 orthologs have evolvedtopossessdistinctactiviti