Function of COP9 Signalosome in Regulation of Mouse Oocytes Meiosis by Regulating MPF Activity and Securing Degradation 英文参考文献.docVIP

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Function of COP9 Signalosome in Regulation of Mouse Oocytes Meiosis by Regulating MPF Activity and Securing Degradation 英文参考文献.doc

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Function of COP9 Signalosome in Regulation of Mouse Oocytes Meiosis by Regulating MPF Activity and Securing Degradation 英文参考文献

FunctionofCOP9SignalosomeinRegulationofMouse OocytesMeiosisbyRegulatingMPFActivityand SecuringDegradation EunjuKim1,Se-JinYoon2,Eun-YoungKim3,YunnaKim1,Hyun-SeoLee1,Kyeoung-HwaKim1 ,Kyung-Ah Lee1,3 * 1DepartmentofBiomedicalScience,CollegeofLifeScience,CHAUniversity,Seoul,Korea,2DepartmentofGenetics,StanfordUniversitySchoolofMedicine,Stanford, California,UnitedStatesofAmerica,3CHAResearchInstitute,FertilityCenter,CHAGeneralHospital,Seoul,Korea Abstract TheCOP9(constitutivephotomorphogenic)signalosome(CSN),composedofeightsubunits,isahighlyconservedprotein complexthatregulatesprocessessuchascellcycleprogressionandkinasesignalling.Previously,wefoundtheexpression oftheCOP9constitutivephotomorphogenichomologsubunit3(CSN3)andsubunit5(CSN5)changesasoocytesmaturefor thefirsttime,andthereisnoreportregardingrolesofCOP9inthemammalianoocytes.Therefore,inthepresentstudy,we examinedtheeffectsofRNAinterference(RNAi)-mediatedtransientknockdownofeachsubunitonthemeioticcellcyclein miceoocytes.FollowingknockdownofeitherCSN3orCSN5,oocytesfailedtocompletemeiosisI.Thesearrestedoocytes exhibited a disrupted meiotic spindle and misarranged chromosomes. Moreover, down-regulation of each subunit disrupted the activity of maturation-promoting factor (MPF) and concurrently reduced degradation of the anaphase- promoting complex/cyclosome (APC/C) substrates Cyclin B1 and Securin. Our data suggest that the CSN3 and CSN5 are involvedinoocytemeiosisbyregulatingdegradationofCyclinB1andSecurinviaAPC/C. Citation:KimE,YoonS-J,KimE-Y,KimY,LeeH-S,etal.(2011)FunctionofCOP9SignalosomeinRegulationofMouseOocytesMeiosisbyRegulatingMPFActivity andSecuringDegradation.PLoSONE6(10):e25870.doi:10.1371/journal.pone.0025870 Editor:SueCotterill,St.GeorgesUniversityofLondon,UnitedKingdom ReceivedJanuary30,2011;AcceptedSeptember13,2011;PublishedOctober3,2011 Copyright:?2011Kimetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andrepr