Galectin-1-Binding Glycoforms of Haptoglobin with Altered Intracellular Trafficking, and Increase in Metastatic Breast Cancer Patients 英文参考文献.docVIP

下载本文档

5
0
约7.84万字
约 15页
2017-05-11 发布于上海
举报
版权申诉

Galectin-1-Binding Glycoforms of Haptoglobin with Altered Intracellular Trafficking, and Increase in Metastatic Breast Cancer Patients 英文参考文献.doc

1、本文档共15页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Galectin-1-Binding Glycoforms of Haptoglobin with Altered Intracellular Trafficking, and Increase in Metastatic Breast Cancer Patients 英文参考文献

Galectin-1-BindingGlycoformsofHaptoglobinwith AlteredIntracellularTrafficking,andIncreasein MetastaticBreastCancerPatients MichaelC.Carlsson1*,CeciliaCederfur1,VivekaSchaar1,CrinaI.A.Balog2,AdrianaLepur1 ,Franck Touret1,EmmaSalomonsson1,Andre′ M.Deelder2,Ma?rtenFerno¨3,Ha?kanOlsson3,ManfredWuhrer2, HakonLeffler1* 1SectionMIG(Microbiology,Immunology,Glycobiology),DepartmentofLaboratoryMedicine,LundUniversity,Lund,Sweden,2BiomolecularMassSpectrometryUnit, DepartmentofParasitology,LeidenUniversityMedicalCenter,Leiden,TheNetherlands,3DepartmentofOncology,LundUniversityHospital,Lund,Sweden Abstract Serafrom25metastaticbreastcancerpatientsand25healthycontrolsweresubjectedtoaffinitychromatographyusing immobilized galectin-1. Serum from the healthy subjects contained on average 1.2mg per ml (range 0.7–2.2) galectin-1 binding glycoproteins, whereas serum from the breast cancer patients contained on average 2.2mg/ml (range 0.8–3.9), with a higher average for large primary tumours. The major bound glycoproteins were a-2-macroglobulin, IgM and haptoglobin. Both the IgM and haptoglobin concentrations were similar in cancer compared to control sera, but the percentageboundtogalectin-1waslowerforIgMandhigherforhaptoglobin:about50%(range20–80)incancerseraand about 30% (range 25–50) in healthy sera. Galectin-1 binding and non-binding fractions were separated by affinity chromatography from pooled haptoglobin from healthy sera. The N-glycans of each fraction were analyzed by mass spectrometry,andthestructuraldifferencesandgalectin-1mutantswereusedtoidentifypossiblegalectin-1bindingsites. Galectin-1bindingandnon-bindingfractionswerealsoanalyzedregardingtheirhaptoglobinfunction.Bothweresimilarin forming complex with haemoglobin and mediate its uptake into alternatively activated macrophages. However, after uptaketherewasadramaticdifferenceinintracellulartargeting,withthegalectin-1non-bindingfractiongoingtoaLAMP-2 positive compartment (lysosomes), while the galectin-1 binding fractio