Gap Junction Mediated Intercellular Metabolite Transfer in the Cochlea Is Compromised in Connexin30 Null Mice 英文参考文献.docVIP

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Gap Junction Mediated Intercellular Metabolite Transfer in the Cochlea Is Compromised in Connexin30 Null Mice 英文参考文献.doc

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Gap Junction Mediated Intercellular Metabolite Transfer in the Cochlea Is Compromised in Connexin30 Null Mice 英文参考文献

GapJunctionMediatedIntercellularMetaboliteTransfer intheCochleaIsCompromisedinConnexin30NullMice QingChang1,WenxueTang1,ShoebAhmad1,BinfeiZhou1,XiLin1,2* 1DepartmentofOtolaryngology,EmoryUniversitySchoolofMedicine,Atlanta,Georgia,UnitedStatesofAmerica,2DepartmentofCellBiology,EmoryUniversitySchool ofMedicine,Atlanta,Georgia,UnitedStatesofAmerica Abstract Connexin26(Cx26)andconnexin30(Cx30)aretwomajorproteinsubunitsthatco-assembletoformgapjunctions(GJs)inthe cochlea.Mutationsineitheroneofthemarethemajorcauseofnon-syndromicprelingualdeafnessinhumans.Becausethe mechanismsofcochlearpathogenesiscausedbyCxmutationsareunclear,weinvestigatedeffectsofCx30nullmutationon GJ-mediatedionicandmetaboliccouplinginthecochleaofmice.Anovelflattenedcochlearpreparationwasusedtodirectly assessintercellularcouplinginthesensoryepitheliumofthecochlea.Double-electrodepatchclamprecordingsrevealedthat the absence of Cx30 did not significantly change GJ conductance among the cochlear supporting cells. The preserved electricalcouplingisconsistentwithimmunolabelingdatashowingextensiveCx26GJsinthecochleaofthemutantmice.In contrast,dyediffusionassaysshowedthattherateandextentofintercellulartransferofmultiplefluorescentdyes(includinga non-metabolizableD-glucoseanalogue,2-NBDG)amongcochlearsupportingcellswereseverelyreducedinCx30nullmice. Sincethesensoryepitheliuminthecochleaisanavascularorgan,GJ-facilitatedintercellulartransferofnutrientandsignaling moleculesmayplayessentialrolesincellularhomeostasis.Totestthispossibility,NBDGwasusedasatracertostudythe contributionofGJsintransportingglucoseintothecochlearsensoryepitheliumwhendeliveredsystemically.NBDGuptakein cochlearsupportingcellswassignificantlyreducedinCx30nullmice.ThedecreasewasalsoobservedwithGJblockersor glucosecompetition,supportingthespecificityofourtests.ThesedataindicatethatGJsfacilitateefficientuptakeofglucosein thesupportingcells.Thisstudyprovidesthefirstdirectexperimentalevidenceshowingthatthetransferofmetabolically- importantmolecul